four pieces of dialysis tubing in the water for at least 10 minutes.2. After 10 minutes, remove one piece of tubing from the beaker. Use your thumb and index finger to rub the dialysis tubing between your fingers. This will open the dialysis tubing. 3. Wrap one of the yellow rubber bands around one end of the dialysis tubing. Leave approximately 1 – 2 inches of tubing below the rubber band. Tie a knot in the remaining dialysis tubing just above or just below the rubber band. This will create a seal and ensure that the solution will not leak out of the tube later in the experiment.4. To test that no solution can leak out, add a few drops of water to the tubing and look for water leakage. If any water leaks, tighten the rubber band and/or knot in the tubing. Make sure you pour the water out of the tubing before continuing to the next step.5. Repeat Steps 2 – 4 with the three remaining dialysis tubes and the three remaining rubber band colors.6. Reconstitute the sucrose powder (60 g of sucrose in a chemical bottle) by adding 160 mL of warm water and shaking until mixed. This will create 200 mL of a 30% stock sucrose solution. 7. Refer to Table 5 to create sucrose solutions that are 30%, 15%, and 3% concentrated. Use the graduated cylinders to measure the liquids and mix them in the waste beakers, labeling each beaker appropriately. Set these solutions aside.