Lab__1b_Viewing_Bacteria_with_Microscope

Remember parfocal using oil immersion view bacteria

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Remember PARFOCAL! Using oil immersion: View bacteria with low power 100xTM (cant see much, but at least get them in your sights) Protection for your high dry (blue band, 400xTM) Drop of oil Oil immersion lens ONLY USE FINE FOCUS ADJUSTMENT!!! When done, use lens paper to clean up your lens and the stage From the Virtual Microbiology Classroom on ScienceProfOnline.com
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When obtaining a bacterial sample from a tube or plate of media do so gently ! The bacteria is growing as a thin film on top of the media! Don’t scrape so hard that you have pieces of agar in your sample! From the Virtual Microbiology Classroom on ScienceProfOnline.com Image: E. col i growing on TSY agar in slant tube and in Petri dish, T. Port If obtaining bacterial sample from slant tubes: - never pick up test tube by the cap. - do NOT set cap down on lab bench - flame neck of the test tube before & after obtaining sample.
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Making a Bacterial Smear & Simple Stain 1 . With a wax pencil, draw 3 dime-sized circles on a slide. 2. Flip slide over so wax is on the bottom. 3. Use DI water dropper to place a very small drop of water inside each circle. * sterilize inoculation loop in microincinerator and let cool * 4. Using inoculation loop , obtain a small sample of Staphylococcus epidermidis from the source plate or tube, as instructor has demonstrated. Swirl loop in circle of water on left. * IMPORTANT!!!: Very gently obtain sample of bacteria from the source plate or tube. The bacterial colonies are found growing on the surface of the TSY agar. DO NOT remove agar with your sample!!
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