(e.)DNA by itself is not visible to the naked eye when it is in solution. How can one visualize DNA fragments in a gel? To visualize DNA in this exercise, what precautions must be taken? (2)GROUP: Analyze your gel. Show your work.(a.)Include a picture of your gel, labeling all components (samples names, DNA bands, ladder, etc.) What is the purpose of -the positive control DNA sample?
Sydney Horton Sjh2643 Group Members: Shruthi, Taylor, MahaExercise: 4Date: 2/11LI: SanjeevUnique: 49150Day: TRoom: PAI 1.08Lab Start Time: 6 PMExercise 4 Page 3of 9(b.)Prepare a standard curve showing the relationship between known size (in bp) of the molecular weight marker/ fragments (bands of the MW ladder) and the migration distance for each of the bands. Use data from the group’s agarose gel to graph a scatter plot in MS Excel. Place the logarithm of each of the DNA marker sizes (in bp) along the Y-axis of the graph and the migration distances on the X-axis. Perform a linear regression analysis and include the line equation and regression coefficient (R2). (c.)Using the linear regression equation, estimate the approximate size of each of the DNA band(s) found in each of the samples.
Sydney Horton Sjh2643 Group Members: Shruthi, Taylor, MahaExercise: 4Date: 2/11LI: SanjeevUnique: 49150Day: TRoom: PAI 1.08Lab Start Time: 6 PMPage 4of 9(d.)Interpret your results. Compare the size of the bands in the “cut” (+) and “uncut” (–) lanes. Did you see any evidence of supercoiled plasmids or nicked/relaxed plasmids? Were your restriction digestion reactions successful? What is the total size of the pLAFplasmid? How many base pairs (bp) are between the EcoRIand BpmIrestriction sites? Be sure to use the data from your gel to explain your answer.