Materials 96 well microplate distilled water

Materials
96-well Microplate distilled water
toothpicks apron
goggles
microtip pipets
beaker
Procedure
Part I
1. In this activity you will observe what happens when solutions of eight substances are mixed two by two.
Before you begin, form a hypothesis about how you could use these observations to identify unknown
solutions of the same substances. Record your hypothesis under Data and Observations.
2. Place a 96-well microplate on your lab bench so that the numbered columns are at the top and the lettered
rows are at the left.
3. Assign one of the eight known solutions to each of rows A-H. silver nitrate, for example, might be in row A,
silver nitrate in row B, and so on. Set up a data table by writing the name of each solution next to the letter of
its row. Part of a labeled data table is shown in Table 1 under Data and Observations.
4. In the same order as in step 3, assign a known solution to each of columns 1-8. Write the names above the
columns in the data table.
5. The labels on your data table show the solutions to be mixed in each square. For example, if your data form
matched Table 1 in Data and Observations, you would mix silver nitrate and nitric acid in well B1. To avoid
wasting materials, do not mix a substance with itself and do not mix the same two substances more than
once. Place X's in the need to mix solutions.
6. Using a clean microtip pipette, place 3 drops of the solution you assigned to row B onto the plastic wrap
over each square in row B that does not have an X. Caution: Hazard the solutions with care. Nitric acid and
sodium hydroxide can burn the skin and make holes in clothing. Silver nitrate can stain the skin black. Once