this scenario, once the eye progenitor was removed, this absence was ‘sensed’ in some mannerby the embryo and led to regulatory events that effectively replaced the missing tissue. A mosaicmechanism would be suggested if removal of the eye progenitor at this particular developmentalstage led to an absence of an eye; in this case, the future eye is autonomous from the rest of theembryo, suggesting that only the eye progenitor is capable of becoming eye (perhaps through theinheritance of particular determinants).6.What technology could you use to test if your chosen eye progenitor gene is necessary for eyeformation. Clearly state one possible phenotype that you would predict if your eye is necessary,one possible phenotype if your gene is not necessary.You could knockdown the eye progenitor gene using a technology such as antisense morpholinoknockdown. To do this you would inject the morpholino oligonucleotide into the fertilized embryoand then look at the effect on eye development. If your gene is necessary, you might expect tofind that the eyes of injected embryos fail to form; conversely, if your gene is not necessary, youmight expect to see that eye development is completely normal.
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