D without touching the inoculation loop to anything

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alcohol, keep it angled down so that no alcohol drips back onto your hand. d. Without touching the inoculation loop to anything, carefully pass the end of the inoculation loop through the flame several times. e. Extinguish the flame when complete. 6. Remove about half of the first colony from the plate by scraping it up with an inoculation loop. 7. Remove the lid from one of the agar plates prepared in Step 1. Remember to hold the lid closely over the top of the plate to prevent airborne contamination of the plate. 8. Gently streak the inoculation loop over the surface of the agar in a zig-zag pattern, taking care to not dig into the surface of the agar with the loop. 9. Place the lid on the plate, and seal it with Parafilm ® .
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10. Turn the plate upside-down, and label it as “Plate #1” with your permanent marker. 11. Use a sterile inoculation needle to remove the other half of the first colony. 12. Stab/push the needle approximately half-way into one of the stab tubes. 13. Pull the inoculating needle straight up and out through the same path that it went down into the media. Dispose of the needle. 14. Snap the lid on the tube, and label the tube as “Tube #1” with your permanent marker. Be sure to press the lid all the way down to create an airtight seal. 15. Repeat Steps 5 – 14 with two other selected colonies from your reserved plate. Label the plates and tubes as “Plate #2” “Tube #2”, “Plate #3”, and “Tube #3”, respectively. 16. Repeat Step 5 and gently streak your freshly sterilized inoculation loop across the fourth plate without touching the loop to any other surface. Place the lid on the plate, and seal it with Parafilm
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  • Fall '19
  • Agar plate, Petri dish, Agar, Stab Tube

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