Lab__3b_Identification_of_Unknown_Bacteria_-_Part_II

Bacillus subtilus from the virtual microbiology

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Bacillus subtilus From the Virtual Microbiology Classroom on ScienceProfOnline.com Intense pink Light, uneven pink
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API – 20E The stains and media that we use to help ID bacteria are mainly useful for preliminary ID, not usually to the species level. Once we have some idea as to the identity of a bacteria, we can then perform more sensitive tests for more specific identification. The API-20E test is used to ID Gram-negative bacilli from the family Enterobacteriacea. Q: So what test would someone need to do on a bacterial sample before utilizing the API- 20E? The API-20E is a system of 20 individual, miniaturized tests used to determine the metabolic capabilities of the organism. From identification of metabolic capabilities, we can zero in on the identification of the genus and species. The tests allow us to come up with a numerical 7-digit profile, based on which tests are positive and which are negative. You then look up that magic number, to find the species identification of your sample. Images: API-20E with results sheet, T. Port From the Virtual Microbiology Classroom on ScienceProfOnline.com
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Image: API-20E results for different species of bacteria, Dr. Charles Gerba , University of Arizona From the Virtual Microbiology Classroom on ScienceProfOnline.com
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Now go be a bacteria detective! In order to figure out what organism your unknown is, you will need to… 1. Examine your Gram , Acid fast and Endospore stain results. 2. Examine the MacConkey’s and Mannitol Salt plates that you inoculated last week. 3. Use the dichotomous key I have placed on your bench to figure out what your unknown is. 4. Once you have identified your unknown, write the unknown number and its scientific name on your Lab Report 3b. From the Virtual Microbiology Classroom on ScienceProfOnline.com
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Gram Stain Examples Staphylococcus epidermidis Escherichia coli Mixed Sample of S. epidermidis & E. coli Images: All Gram stain images by T. Port From the Virtual Microbiology Classroom on ScienceProfOnline.com
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Acid Fast Stain Examples Mixed sample of Mycobacterium smegmatis & Micrococcus luteus Mycobacterium smegmatis Images: All acid fast stain images by T. Port From the Virtual Microbiology Classroom on ScienceProfOnline.com
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