5 wv sodium chloride but d grow at pH 96 Isolates attack produce acid from N

5 wv sodium chloride but d grow at ph 96 isolates

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37°C but not at 10 or 45°C, or in 40% bile or 6.5% (w/v) sodium chloride, but do grow at pH 9.6. Isolates attack (produce acid from) N-acetyl-glucosamine, aescu- lin, arbutin, cellobiose, D-fructose, gentiobiose, D-glucose, glycogen, maltose, mannitol, D-mannose, melezitose, ribose, salicin, starch and trehalose, but not adonitol, amygdalin, L- or D-arabinose, L- or D-arabitol, dulcitol, erythritol, L- or D-fucose, galactose, gluconate, glycerol, inositol, inulin, lactose, melibiose, D- raffinose, rhamnose, sorbitol, L-sorbose, tagatose, turanose, xyHtol, or L- or D- xylose. Alkaline phosphatase, arginine dihydrolase, P-glucuronidase, leucine aryl- amidase and pyrrolidonylarylamidase are produced, but not a- or P-galactosidase. The Voges Proskauer reaction is negative, oc-haemolysis is recorded for bovine blood (Eldar et al., 1994). By serology using streptoccocal-specific antisera, the original isolates equated with Str. shiloi were untypeable. Moreover, these isolates were considered to belong to a separate and distinct DNA homology group, with DNA relatedness between mem- bers of 89-100% (Eldar et al., 1994). Some phenotypic differences were noted
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62 Bacterial Fish Pathogens between fish and human isolates; however, molecular techniques did not discriminate the two sets of cultures (Dodson et ai, 1999). Streptococcus milled Two cultures were obtained from kidney samples in ulcerated Koi carp (Austin and Robertson, 1993). The following characteristics were displayed: Streptococcus millcri Cultures contain catalase- and oxidase-negative fermentative cocci in chains, that produce acid and alkaline phosphatase, arginine dihydrolase, chemotrypsin, ester- ase (caprylate and lipase), P-galactosidase, leucine and valine arylamidase and pyrrolidonylarylamidase but not cystine arylamidase, a-fucosidase, ot-galactosi- dase, a- and P-glucosidase, P-glucuronidase, H2S, indole, ot-mannosidase, nitrate reductase, trypsin or tryptophan deaminase. The methyl red and bile aesculin tests are negative. Aesculin, arginine, casein and horse blood (weakly P-haemolytic) are degraded, but not DNA, gelatin, sodium hippurate, starch or urea. Growth occurs in 0-1.5% but not 8% (w/v) sodium chloride and on MacConkey agar. Citrate is utiHsed. Acid is produced from N-acetyl-glucosamine, amygdalin, D-fructose, D- glucose, inositol, maltose, D-mannose, ot-methyl-D-glucoside, ribose, saccharose, sucrose, D-trehalose, xylitol and xylose, but not from L-arabinose, glycogen, inulin, lactose, D-mannitol, D-melibiose, raffinose, rhamnose or sorbitol. An identification to Str. millcri (probability of a correct identification = 98%) resulted from use of the Bacterial Identifier Program (Bryant and Smith, 1991). By means of the API 20 STREP system, an identification of Lactococcus lactis resulted (probability of a correct identification = 96.7%). Yet, from the published description there was a closer fit with Str. millcri. The only discrepancies concerned utilisation of citrate, and acid production from inositol, xyHtol and xylose. The isolates from Koi carp were positive for these tests.
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