Materials and methods the independent variable for

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Materials and Methods: The independent variable for our experimental trial was the change in the pH of H2O2 (6,7,8, and 9). The dependent variable is the time it takes for the reaction to happen (time for the paper to float). The control group was the water. The constants were temperature (25 degrees C), 1.5% H2O2, species type of catalase (cow), and concentration of H2O2 (40U/ml). Result: In graph A it shows the higher the concentration of catalase the faster the reaction will occur. In graph B it shows the lower the percent of substrate (H2O2) the longer it takes for the little paper to float to the top. In graph C it shows the effect of pH has on catalase and pH 7 caused the slowest reaction while the other ones (6,8,9) caused the reaction to happen fast. In graph D it shows that the reaction happens the best at room temperature (25 degrees C). in the last graph the chicken catalase caused the fastest reaction than potato, cow, and yeast. According to the phylogenic tree chicken and cow are mostly related because they have the genetic make-up of active sites and amino acids. While potato and yeast are mostly related because of their amino acids and actives sites and they are all linked to together because of yeast. Even though two of them are plants and two are animals that doesn’t mean that they can’ have some similarities when looking at their alignment, most of the time yeast, chicken and cow would have similarities when potato wouldn’t have that same thing.
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  • Spring '18
  • Dr.Jan Fry
  • Enzyme, Catalase

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