Focus on the specimen using the yellow 10x lens use

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• Focus on the Specimen using the Yellow 10X Lens. • Use the Black Plastic Collar on the Lens Turret to click the Red 3.2X Lens into Place. • Add a Drop of Immersion Oil (in your Wooden Stain Holder) directly to the Slide When you place a Drop of Immersion Oil directly atop the Apex of the Solid Cone of Light being beamed through your Specimen, you ʼ re rewarded with a quick Bling of Light. See how the Glass Rod “disappears” when it ʼ s n the Oil? That ʼ s because Glass and the Oil have exactly the same Refractive Index. • Slowly move the 100X Oil Immersion Lens into the Oil until you feel it click into Place You always have to slightly change the Focus every Time you go to Oil (but never be more than about Half a Turn of the Fine Focus Knob) Your Bacteria will still look very Small, but you should be able to make out the Shape and the Color. Your E. coli should look like little Pink Rods. Your Staphylococcus epidermidis should look like little Purple Spheres. Compare your Slide with your Lab Partner ʼ s Slide. It ʼ s always a Good Idea to compare Results with your Lab Partner, and feel free to compare outside of your Group, too. • If you want to look at another Slide under Oil Immersion, move the Lens Turret to the 3.2X Lens, remove the first Slide, replace it with the other Slide, add a Drop of Immersion Oil and move the 100X Oil Immersion Lens into the Oil until it clicks into Place. • After using Oil Immersion, return to the 3.2X Position. Very gently clean the Oil off the 100X Lens with Lens Paper. The Lens Paper is designed for cleaning the Lenses of Microscopes, which tend to have relatively Soft Lens Coatings. Please don ʼ t use Kimwipes, which are surprisingly Abrasive. (Note: Lens Paper is great for cleaning the LCD Screen of your Digital Camera and iPhone, too.) __________________ “Do you like your army?” I look forward to seeing them in action.’ They'll do their job well. I'll guarantee that.” © 2002 LucasFilm Ltd.
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Lab 3 Page 14 Photomicroscopy Point and Shoot Digital Cameras (because their 1/2.3” Sensors use extremely short Focal Length Lenses) can take surprisingly good Pictures through our Microscopes. Here ʼ s how: • Turn your Camera “On”. • Set your Camera to “P” (not the Green Camera Icon). • Set your Camera to “Flash Off”. This is easy to do on most Cameras. Sony Cyber-shots have a dedicated Flash Mode Button. On other Cameras you can usually access Flash Modes directly from the Menu. On some Cameras your only Option may be to pick a “Night Scene” Mode from your Scene Mode Selection. Figure 3-2. Typical Camera Flash-Off Icons • Leave the Zoom at its Default “Wide” Setting (if you have a Zoom Lens) • Center the Camera Lens over the Microscope Objective. This is the only tricky Part. The Camera Lens needs to be about the same Distance from the Microscope Objective as your Eye is during Normal Use (which is really Close).
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