Cholesterol hydrophobic polar head group differences

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Cholesterol hydrophobic Polar head group Differences in sterols across kingdoms; mainly in eukaryotes Amphipathic Enhances barrier properties Modulates fluidity -temperature dependence -concentration dependence 9
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Membrane Fluidity:Lipids Mobility of Individual phospholipids fatty acid chains can flex individual phospholipids rotate rapid lateral diffusion flip flop is rare 10
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Membrane Asymmetry Sphingolipids are found almost exclusive to the exoplasmic leaflet PS and PI are negatively charged under physiological conditions; localized to cytoplasmic leaflet PC and PE have no net charge and are found in both leaflets depending on the cell type Cholesterol has equal distribution in both leaflets PC, sphingomyelin, glycolipids PS and PI 11
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Proteins in membrane Integral 1 - Single-pass 2 - Multi-pass Integral Peripheral Exo Cyto Peripheral 3 - Fatty acid chain attachment to membrane 4 - Oligosaccharide-linked attachment to membrane 5, 6 - Noncovalent attachment to protein anchor 12
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Membrane Fluidity: FRAP Study the movement of membrane components Fluorescence Recovery After Photobleaching Free diffusion of labeled molecule- faster recovery Membrane anchored protein- little to no recovery 13
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Membrane Fluidity: FRAP 14
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