Moraxella sp Pure culture growth of round raised translucent mucoid colonies

Moraxella sp pure culture growth of round raised

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Moraxella sp. Pure culture growth of round, raised, translucent, mucoid colonies developed in 48 h of incubation at 22° C of kidney, liver and pancreas tissue on TSA (Baya et al, 1990b). Moritellaceae representatives Moritella marina Benediktsdottir et al. (1998) used 5% (v/v) horse blood agar supplemented with 1.5% (w/v) sodium chloride with incubation at 15°C for 7 days. Neisseriaceae representative Aquaspirillum sp. Lio-Po et al. (1998) used TSA supplemented with 10% (v/v) horse serum and cytophaga agar at an unspecified temperature and duration to recover Aquaspirillum, Aer. hydrophila and Streptococcus from diseased animals. Oxalobacteraceae representative Janthinobacterium lividum Homogenates of whole fish (prepared in quarter-strength Ringer's buffer; Oxoid) and, where possible, loopfuls of kidney, liver, spleen, ascitic fluid and material from surface lesions were spread over the surface of a variety of media, including blood agar (5% v/v bovine blood in Gibco blood agar base), cytophaga agar, KDM2, L-F medium (Appendix 5.1) and TSA, with incubation aerobically at 22°C for up to 14 days. Purple-pigmented colonies were apparent after 3 days. Pasteurellaceae representative Pasteurella skyensis Isolation from kidney was on TSA supplemented with 1.5% (w/v) sea salts and 5% (v/v) defibrinated horse blood aerobically at 20°C for 48 h when small, grey colonies
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170 Bacterial Fish Pathogens resulted. After initial isolation, culturing was possible on TSA (or Columbia agar) supplemented with 1.5% (w/v) sodium chloride and 10% (v/v) citrated sheep or horse blood (Birkbeck et al, 2002). Photobacteriaceae representatives Photobacterium damselae subsp. damselae Isolation may be readily achieved by swabbing ulcerative material onto the surface of BHIA supplemented with 5% (v/v) sheep blood, or TCBS (Appendix 5.1) with incubation at 25°C for an unspecified period (probably 2-5 days) (Fujioka et al, 1988; Sakata et al, 1989; Fouz et al, 1991). Photobacterium damselae subsp. piscicida The organism may be isolated by inoculating swabs of kidney and/or spleen material onto marine 2216E agar (Difco), nutrient agar or blood agar, with incubation at 25°C for 48-72 h. An improved Hquid medium has been described, which may be soHdified by the addition of 1 % (w/v) agar (Appendix 5.1; Hashimoto et ai, 1989). On conven- tional media, shiny, grey-yellow, entire, convex colonies develop, which are approxi- mately 1-2 mm in diameter after 72 h (Kusuda and Yamaoka, 1972). Another approach, which has met with success, has enabled the recovery of Ph. damselae subsp. piscicida from water in the vicinity of fish. The method involved filtering 250 ml volumes of water through 0.45 |im cellulose nitrate filters, before transfer (of the filters) to 2216E agar supplemented with 1% (w/v) mannitol and 0.5% (w/v) phenol red. The non-fermenting Ph. damselae subsp. piscicida produced red colonies (ReaH et al., 1997). With this method, the pathogen was detected on sea bass 8 days before the outbreak of disease.
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