First, the PT and APTT specimen should be collected since the first response after puncture is to coagulate. The PT and APTT specimens would be collected in a light blue topped collection tube which contains the sodium citrate anticoagulant. The specimen should be inverted 3 to 4 times. I would continue to collect a LDH specimen with a light green topped tube which contains lithium heparin anticoagulant and gel separator since the light green topped tube is next in sequence when collecting for the given specimens. I would also invert this sample several times. Then, I would proceed to collect the CBC specimen in the purple EDTA tube. I would invert this specimen several times. Next, I would collect the glucose specimen in a gray topped blood tube which could contain sodium fluoride or potassium oxalate anticoagulant. This would also be inverted several times. Next, I would removed the needle from the patient’s arm, apply gauze to stop the bleeding, and apply a band aid. I would also label the tubes with the appropriate information such as the patient’s name, the patient’s identification number or code, the day and date of collection, and my identification code. 7. I would proceed to perform a manual WBC differential since a CBC does not account for the amount of each individual type of WBC which is significant in diagnosing. Before, preparing a slide for a manual count, I would thoroughly mix the sample since sedimentation will have occurred and the patient may have a disorder that affects the sedimentation rate and if not mixed this may obscure the actual condition of the patient since the cells will not be dispersed evenly throughout the sample.
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