The α naphthol will oxidize the acetoin to diacetyl

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The α -Naphthol will oxidize the Acetoin to Diacetyl, which in turn will react with Guanidine (present in the Tryptone) to produce a Red Color. E. coli is Voges-Proskauer Negative. The MR-VP Broth will not change Color when you add α -Naphthol and KOH. Enterobacter is Voges-Proskauer Positive. The MR-VP Broth will change Color to what looks like Grenadine over Triple-Sec when you add α -Naphthol and KOH.
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Lab 6 Page 29 Citrate (Atlas Page 64) “Can this Bacterium use Citrate as its sole Carbon and Energy Source?” The Citrate Test is designed to determine whether a Bacterium can utilize Citrate as its Sole Carbon Source. Citrate Agar contains Citrate in the Form of Sodium Citrate. As the Sodium Citrate is utilized, CO 2 will accumulate and react with Na + and H 2 O to form Alkaline Compounds. This raises the pH of the Medium so the Bromthymol Blue pH Indicator will change from a Cool and Refreshing Coastal Redwoods Green to an Offshore Deep Ocean Malibu Blue. E. coli is Citrate Negative and so the Slant will remain Redwoods Green. Enterobacter is Citrate Positive and so the Slant will turn Malibu Blue. Citrate Slants aren’t just Fun; they’re also Esthetically Pleasing (particularly after Incubation). You inoculate a Citrate Slant using your Loop to streak down the Surface of the Slant (not a Stab). The Utilization of Citrate is an Aerobic Process, so there’s no need to unleash your Inner Samurai Microbiologist. The Citrate Test is a very Reliable Biochemical Test. ______________ “You could've kept it.” ‘They'd just put it in the museum, it'd be another rock collecting dust.’ “But then it would have given you your fortune and glory.” ‘Anything can happen. It's a long way to Delhi.’ No, thanks. No more adventures with you, Dr. Jones.” Sweetheart, after all the fun we've had together?’ If you think I'm going to Delhi with you, or anyplace else after all the trouble you've gotten me into, think again, buster! I'm going home to Missouri where they never feed you snakes before ripping your heart out and lowering you into hot pits! This is not my idea of a swell time! Excuse me, sir. I need a guide to Delhi. If you could... Oh...” Very funny. Very funny. Uh-oh! © 1984 LucasFilm, Ltd.
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  • Winter '09
  • MANN
  • Microbiology, Bacteria, Escherichia coli, gram-negative bacteria, nutrient agar plates, MacConkey Agar Plates

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