100%(1)1 out of 1 people found this document helpful
This preview shows page 19 - 22 out of 39 pages.
- Moraxella - Acinetobacter - Veillonella Metabolism - oxidizes glucose (only) to acids - fastidious > capnophilic (4-8% CO2) > will notgrow at 22oC; prefers 30-37oC > susceptible to cold, heat & drying Other Unique Features - *NATURAL TRANSFORMER* > highly autolytic @ stationary phase * know mechof invasion!!! Virulence - nocapsule - pili: antiphagocytic & attach to columnar epi > necessary for DNA uptake (transformation) > exhibit antigenic variation - porins (Por A, B) translocate to host membranes to induce epi cells to ingest them - Rmp(a surface protein) > Ag for prod of IgG blocking AbV(anti IgM, anti C’) - lipooligosaccharide (LOS)Vdamages cilia → sloughing> exhibits antigenic variation > target of IgM antibody- IgA proteaseV - steals Fe+from transferrin, lactoferrin & Hb Opa: adherence > antigenic var (+) = opaque, local disease (-) = transparent, Disseminated GI porinscan be used for serotypingb/c invariableCOMPAREto N. meningitidis Transmission - venereal infxn usu. acquired via GU epithelium- Risk factors: ↑sexual partners, age (peaks at 15-24, 25-30), blocking Ab, terminal C’ factor deficiency- endemic diseaseAssociated Diseases In ♂* Urethritis(only ~10% aSx) * EpididymitisIn ♀(50% are aSx) * Cervicitis* Salpingitis* Pelvic Inflammatory Disease(15-20%) * Disseminated gonococcal infection (rare) - polyarthralgia, tenosynovitis, dermatitis/septic arthritis - pustular, erythematous lesions Future test: nucleic acid hybridizationDiagnosis ♂: GC on Gram stain is diagnostic ♀: need culture of cervical pus to make diagnosis In ♀use Thayer Martin media, kill off normal flora Treatment(s) * ß-lactam & tetracycline resistant > plasmid-encoded ß-lactamase > chromosomally-mediated ↓in OM permeability * Cephtriaxone(IM), Cefixime(oral) ora quinolone > single dosefor better patient compliance
Neisseriadiplococci inside of WBCs from peripheral blood smear
More to come next quarter Chlamydia trachomitisLab IDlittle or no cell wall(but may be considered G-) Metabolism - “Energy parasite” (cannot make ATP) > imports nutrients from cytosol into endosome via tubular projections (like a Type III secretory system) Other Unique Features OBLIGATE INTRACELLULAR PARASITE > prevents phago-lysosome fusion > lives in phagosome- Life Cycleinvolves: > elementary body (EB): roundw/strong spore-like coat > reticulate body (RB): replicative form; analogous to bacterial cell - causes persistent and recurrent infxns -EBattaches to host R →parasite specified endocytosis -EBdifferentiates into RB→ binary fission -condense DNA, disulfide bridge of MOMP → EBsare releasedVirulence Transmission - 1ohost = humans - main vector = common house fly> disease of underprivileged w/poor hygiene - 2 biovars (based on target cells) > biovar trachomasquamocolumnar cells of eye & GU tract, conjunctivitis in primates > biovar lymphogranuloma venereum (LBV)infects inguinal LNs →LV Chronic conjunt Serovars A, B, C Oculogenital dis Serovars D, E, F, G,( B, C, H, I, K) Injection of LBVintracerebrally in mice is lethal!