High temperatures denature the enzyme by breaking its

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Temperature changes the confirmation of the enzyme. High temperatures denature the enzyme by breaking its hydrogen bonds and alters the confirmation permanently which does not allow the enzyme to function. Lower temperatures slow the collision of the substrate molecules and decrease the rate of the formation of products. III. The effect of substrate availability on enzyme activity Results:
10 Min 20 Min 30 Min Tube #1 Purple Purple Purple Tube #2 Purple Purple Purple Tube #3 Light Purple Purple Light Purple / Light Pink Tube #4 Dark Purple Purple Dark Purple Answers: 1. Tubes with bile changed color faster as bile helps with digestion and emulsification of fats which will cause Tube 3 to change color faster. 2. Bile emulsifies fats and arranges them into micelles which increase the surface area to allow pancreatin to digest the oil quicker. 3. Tubes #2 and #3 with pancreatin would eventually turn pink because Pancreatin can still work to produce fatty acids from triglycerides however it would be a slow process. Tube #1 and #4 would remain dark as they do not have the enzymes necessary to digest fats.

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