15 Who was the developer of DNA arrays Why do cDNAs stick to the glass slide in

15 who was the developer of dna arrays why do cdnas

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15. Who was the developer of DNA arrays? Why do cDNAs stick to the glass slide in the large-scale expression studies? Pat Brown was the developer of the DNA arrays. cDNAs stick to the glass slide because the slides are coated with poly- lysine, which is positively charge. DNA is negatively charge so the DNA sticks to the slide through an ionic interaction. 16. What disease was tested with the arrays? What was found? How many cDNA genes were printed onto one lymphochip ? Cancer of the lymph nodes and found subtypes of DLBCL. That correlated with survival rates. Total of 17,856 cDNA genes were printed onto one lymphochip. 17. Does each square on the gene chip (glass slide) correspond to a different cDNA? Yes because each square on the gene chip (glass slide) correspond to a different cDNA. 18. What kinds of tags and colors were used to label different sets of cDNA? Labeled one set of cDNA with red fluorescent tag, and the other with the green tag. 19. How could the level of gene expression be figured out based on the color signal? Describe the procedure and outcome. The arrays were incubated with the tagged cDNAs, which bound to the matching genes printed on the array. If the gene was only expressed in DLBCL1 cells, the square was red. Similarly, if the gene was only expressed in DLBCL2, the square was green. And if the gene was expressed equally in both cells, the square was yellow. 20. What is pharmacogenomics ? What is it based on? What are the other potential benefits of DNA array testing? Pharmacogenomics is the study of how genes affect a person's response to drugs. DNA arrays are used to determine which drugs might be best prescribed for particular individuals, because genes determine how our bodies handle the chemistry related to those drugs. 21. Describe a DNA array. DNA arrays are used to determine which drugs might be best prescribed for particular individuals, because genes determine how our bodies handle the chemistry related to those drugs. 22. What are the major differences between DNA arrays and GeneChip probe arrays? The GeneChip probe arrays is used to build the sequence that you want to screen and GeneChip arrays are printed on special glass. 23. Describe the light-directed chemical synthesis. Here, A stands for Adenine, and X for the protector group. A substrate with a nucleotide is fixed onto the chip at specific positions. The nucleotide has a protecting group that blocks polymerization. This protector is photolabile and is released on exposure to UV light.
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