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2 remove the agent 3 put organisms in favorable

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2. Remove the agent. 3. Put organisms in favorable growth medium. 4. Look for reproduction of organisms. From the Virtual Microbiology Classroom on ScienceProfOnline.com
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Methods of Microbial Control That We Will Be Examining Physical Heat UV Chemotherapeutic Antimicrobic drugs, like antibiotics Chemical Bleach, other chemical disinfectants From the Virtual Microbiology Classroom on ScienceProfOnline.com
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Effects of High Temperatures Denaturation of proteins • Interference with integrity of cytoplasmic membrane and cell walls • Disruption of structure and function of nucleic acids Boiling - Kills vegetative cells of bacteria, protozoa and fungi, and most viruses in 10 minutes (at sea level). - Boiling time is critical. - Some heat is lost as steam. - Endospores, protozoan cysts, and some viruses can survive boiling Autoclaving - Pressure applied to boiling water prevents steam from escaping. - Boiling temperature increases as pressure increases. - Autoclave conditions: 121ºC (~250 o F), 15 psi, 15 minutes. Image: Autoclave , Astell Scientific From the Virtual Microbiology Classroom on ScienceProfOnline.com
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A: Microbial Control Using Heat Each pair of lab partners should inoculate 4 tubes of nutrient broth with E. coli and 4 tubes with Bacillus species (spp.). Label the tubes clearly indicating which microbe they contain and include your initials. You will be exposing each tube of E. coli and each tube of Bacillus to different temperatures. Label each of the 4 E. coli tubes with the temperature it will be exposed to: 40 o C, 60 o C, 80 o C, and 100 o C. Label each of the 4 Bacillus tubes with the temperature it will be exposed to: 40 o C, 60 o C, 80 o C, and 100 o C. Place each tube into the appropriate water bath for 10 minutes. Remove the tubes after 10 minutes at temperature. Put them into your “SAVE” test tube rack. 40 60 80 100 From the Virtual Microbiology Classroom on ScienceProfOnline.com
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How to Inoculate a Broth Medium Image: Microincinerator, T. Port; Test tubes, Source unknown From the Virtual Microbiology Classroom on ScienceProfOnline.com
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Methods of Microbial Control That We Will Be Examining Physical Heat Ultra Violet Radiation Sunlight contains the complete spectrum of short to long wavelengths of light. The short, invisible ultraviolet (UV) wavelengths are injurious to non-photosynthetic bacteria. UV radiation is strongly absorbed by proteins and nucleic acids . UV radiation may cause enzyme inactivation, genetic mutation or death. Chemotherapeutic Chemical Image: Sun's corona as seen in deep ultraviolet by the Extreme ultraviolet Imaging Telescope, NASA From the Virtual Microbiology Classroom on ScienceProfOnline.com
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B. Effectiveness of UV Radiation as a Way to Control Bacteria • Use 2 TSY Plates.
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