Animals Plants K BARRETTO FOR BIOL101 LAB ACTIVITIES FROM DOWDY YONENAKA 2018

Animals plants k barretto for biol101 lab activities

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Animals Plants K. BARRETTO FOR BIOL101 | LAB ACTIVITIES FROM DOWDY & YONENAKA 2018
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CATALASE 1. Hydrogen peroxide is bad for your cells 2. Catalase is an enzyme that breaks down harmful H2O2 into harmless water and oxygen How might we observe the breakdown of hydrogen peroxide and activity of catalase? K. BARRETTO FOR BIOL101 | LAB ACTIVITIES FROM DOWDY & YONENAKA 2018 H 2 O 2 Hydrogen Peroxide H 2 O Water O 2 Oxygen Catalase
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QUESTION: WHERE IS CATALASE FOUND? K. BARRETTO FOR BIOL101 | LAB ACTIVITIES FROM DOWDY & YONENAKA 2018
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PROCEDURE 1. Discuss with your group what samples you are going to test and which controls you need (this should take NO MORE than 3 minutes!!) 2. Record which samples have catalase and note the differing degrees of activity that you observe. K. BARRETTO FOR BIOL101 | LAB ACTIVITIES FROM DOWDY & YONENAKA 2018 Name of Sample Type of Sample Reaction Observatio ns Catalase Present or Not Present? Catalase Positive Control DI Water Negative Control Name of Sample Plant (Experimenta l Sample) Name of Sample Animal (Experimenta l Sample)
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PROCEDURE 3. Draw conclusions based on your results. Was your hypothesis correct? Do both plant and animal tissues have an enzyme that breaks down hydrogen peroxide? 4. Be prepared to briefly discuss results with the class. K. BARRETTO FOR BIOL101 | LAB ACTIVITIES FROM DOWDY & YONENAKA 2018
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OPTIMAL CONDITIONS CASE STUDIES Activities 4 & 3 K. BARRETTO FOR BIOL101 | LAB ACTIVITIES FROM DOWDY & YONENAKA 2018
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TALK TO YOUR GROUP! Why can fevers be bad for us? Why is hypothermia (temperature too low) bad for us? K. BARRETTO FOR BIOL101 | LAB ACTIVITIES FROM DOWDY & YONENAKA 2018
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WHAT IS “AMYLASE”? Amylase is an enzyme in your saliva Starch (remember plant storage molecules?) is a big chain of glucose molecules (small sugars) Amylase breaks up starch into smaller sugars K. BARRETTO FOR BIOL101 | LAB ACTIVITIES FROM DOWDY & YONENAKA 2018 Glucos e Glucos e Glucos e Glucos e Starch Amylase Glucos e Glucos e Glucos e Glucos e
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WHAT IS “TENDAMISTA T”? Tendamistat is a chemical (drug) Inhibitor: Something that affects the structure of the enzyme What is the relationship between structure and function? K. BARRETTO FOR BIOL101 | LAB ACTIVITIES FROM DOWDY & YONENAKA 2018
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Activi ty 4 TIME FOR STARCH TO DISAPPEAR VS. PH pH Time for Starch to Disappear (min) 4 7 5 5 6 2 7 6.5 8 8.5 K. BARRETTO FOR BIOL101 | LAB ACTIVITIES FROM DOWDY & YONENAKA 2018 3.5 4 4.5 5 5.5 6 6.5 7 7.5 8 8.5 0 1 2 3 4 5 6 7 8 9 Time for Starch to Disappear vs. pH pH Time for Starch to Disappear (minutes)
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EFFECTS OF TENDAMISTAT Time (min)
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  • Spring '14
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  • Enzyme, Tendamistat

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