BIOL 100 Lab5 Cell Membranes_Transport Diffusion and Osmosis_RPT.rtf

Data table 3 potato mass versus solution bar graph

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Take a photo of all of the test tubes. Data Table 3. Potato Mass versus Solution Bar graph with Sucrose Molarity on X-axis and % Mass Change (positive or negative) on Y-axis (use Excel or hand draw the graph and take a photo) Questions: A) Define osmosis. How did you observe osmosis in this exercise?
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Osmosis is the net movement of solvent molecules through a membrane into a region of higher solute concentration and equalizes the solute concentrations on both sides. Osmosis was observed in this exercise because the sugar solution was less concentrated on the outside rather than the inside there the molecules attempted to equalize this by passing through the potato. B) List molar concentrations (0.0 M, 0.2 M, etc.) at which water entered the potato strips. Why did water move into the potato strips? Were these solutions hypotonic, hypertonic, or isotonic? The molar concentrations at which water entered the potato strips were 0.0M, 0.2M, and 0.4M. As stated earlier, the water moved into the potato strip to try to preform osmosis and equalize the levels of concentration inside as well as outside the potato. This solution was hypertonic. C) List the molarities at which water exited the potato strips. Why did water move out of the potato strips? Were these solutions hypotonic, hypertonic, or isotonic? The molarities at which water exited the potato strips were .8M, and 1.0M. The water moved out of the potato strip to attempt to equalize the concentration levels which makes the solutions hypotonic. D) Was the hypothesis you recorded in Data Table 2 supported or refuted by your experimental results? Discuss your results in relation to your hypothesis. (It is acceptable if your hypothesis was refuted, as long as you can explain WHY.) The hypothesis that I recorded in data table 2 was supported because I had predicted that the higher the sucrose molarities, the more mass they would lose after I had completed this lab and they did. E) Use your bar graph ( Data Table 3 ) to estimate the solution molarity that would be isotonic to the potato and explain your reasoning.
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  • Spring '16
  • Laura Harkewicz, Ph.D.
  • Osmosis, 0.6m, 0.4m, 0.2M, 0.0M

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