Drosophila But poor study design only as good as their controls UASGAL4 system

Drosophila but poor study design only as good as

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Drosophila But poor study design (only as good as their controls…) UAS/GAL4 system UAS = upstream activating sequence GAL4 = a transcription factor protein which binds and activates expression of a gene under control of the UAS Crossed two parental lines of Drosophila , one with UAS+SOD1, the other with GAL4 Progeny demonstrated prolonged lifespan Flawed controls: the parental lines Did not control for hybrid vigor - the hybrid offspring between two inbred parental lines tend to be stronger and demonstrate greater growth due to greater genetic differences Need to test for whether increased expression of SOD1 or hybrid vigor is responsible for prolonged lifespan Inducible expression system - addition of exogenous factor prompts gene expression of target gene Basically found that increased antioxidant defense expression inconsistently affected lifespan In mice, overexpression of either SOD1, catalase, or both do not prolong lifespan FRTA may be interconnected with some hallmarks of aging: Mitochondrial dysfunction Loss of proteostasis Let’s explore mitochondrial dysfunction as a factor for aging Test hypothesis that decline of ETC function resulting in differing ATP
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  • Spring '18
  • David Walker

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