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01.Isolations

And now your highness we will discuss the location of

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And now, your highness, we will discuss the location of your hidden rebel base. .. © 1977 LucasFilm Ltd.
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Lab 1 Page 9 Streaking a Plate (Reader ʼ s Digest Condensed Version): Procedure: 1. Make a “T” on the Bottom of your Petri Plates and label each Plate appropriately. 2. Sterilize your Loop. 3. Cool your Loop in an Empty Area of the Mother Plate 4. Touch the Edge of an Isolated Colony with your Loop. 5. Streak these Bacteria onto the “A” Section of your Plate. 6. Re-sterilize your Loop. 7. Streak through your “A” Section once and proceed to streak your “B” Section 8. Re-sterilize your Loop. 9. Streak through your “B” Section once and proceed to streak your “C” Section. 10. Replace your Petri Plate’s Lid and invert the Plate. 11. Re-sterilize your Loop and return it to its Holder. ___________________________________________________________ “What of the Rebellion? If the Rebels have obtained a complete technical reading of this station, it is possible, however unlikely, they might find a weakness and exploit it.” ‘The plans you refer to will soon be back in our hands.’ Any attack made by the Rebels against this station would be a useless gesture, no matter what technical data they have obtained. This station is now the ultimate power in the universe! I suggest we use it! ‘Don't be too proud of this technological terror you've constructed. The ability to destroy a planet is insignificant next to the potential of the Force.’ Don't try to frighten us with your sorcerous ways, Lord Vader. Your sad devotion to that ancient Jedi religion has not helped you conjure up the stolen data tapes, or given you enough clairvoyance to find the rebels' hidden fortress. .. ‘I find your lack of faith disturbing.” © 1977 LucasFilm Ltd.
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Lab 1 Page 10 Isolations Isolation of Pseudomonas (Enrichment) Materials [per Student] • Sterile 16mm Test Tube • Sodium Benzoate Broth • Soil Sample Procedure 1. Swirl the Sodium Benzoate Broth and add ~5 ml (~ 3 Fingers) of this Sodium Benzoate Broth to a labeled 16mm Test Tube. 2. Add a very small Pinch of Soil to the Sodium Benzoate Broth in your Test Tube. (A “pinch” is less than 0.5 g.) 3. Incubate your Test Tube at 25 ° C until the next Lab. Isolation of Bacillus (Direct Isolation and Selection) Materials [per Student] • Sterile 16mm Test Tube • Test Tube Rack • Distilled Water • Soil Sample • 80°C Water Bath (the steaming one over by the Windows) • Nutrient Agar Plate Procedure 1. Add ~ 5 ml (Three Fingers) of non-sterile, De-Ionized Water to a (labeled!) Sterile Test Tube. 2. Add a Pinch of Soil to the Test Tube and Finger Vortex the Soil Suspension 3. Pasteurize the Soil Suspension in an 80 ° C Water Bath for 20 Minutes. Remember: 80°C is really Hot! 4.
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And now your highness we will discuss the location of your...

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