Remember if the recombinational frequency is below 50

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Remember if the recombinational frequency is below 50% the gene and probe are linked. If it is above 50% then the gene and probe are not linked. Once you have found a probe that is linked to the gene you need to find its exact location by determining the recombinational frequency between the gene and the other probes on the chromosome. The key to this lab is determining recombinational frequencies. This will be a little more difficult than you might think. The reason for this is you do not know how the probe will be linked to the gene (i.e the dominant allele for the gene will not necessarily be linked to the + probe). You must chose pedigrees where you can clearly tell how the genes would be linked in the second generation. This will take some thinking on your part. Procedure: 1. See Mr. Foret for the disease you will be investigating. 2. Go to biologylabsonline.com and log onto pedigree lab. 3. Determine the inheritance of the gene by analyzing numerous full pedigrees for that disease. You must analyze a large number of pedigrees so that you are certain about the inheritance of your disease. If your conclusion is incorrect the rest of your work will be incorrect. 4. Once you know the inheritance of your gene Click on the large family tab. Then select the RFLP probe you want to investigate. Then answer yes to the three questions. There will be three questions about the type of pedigrees you want to search for in the database. Answer the three questions (usually you want to answer yes to all three questions). Then click the search database box.
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