by two experienced pathologists and diagnosed as sarcomas which invade multiple

By two experienced pathologists and diagnosed as

This preview shows page 7 - 8 out of 8 pages.

by two experienced pathologists and diagnosed as sarcomas, which invade multiple organs. Magnification: all 40 6 . (TIF) Acknowledgments We gratefully acknowledge the technical support of Ken Harpal for histological analysis. We also thank Dr. Andrew P. McMahon at Harvard Medical School for providing us with Six2-Cre mice. Author Contributions Conceived and designed the experiments: SEQ. Performed the experi- ments: YM MB CL. Analyzed the data: YM PT SEQ. Contributed reagents/materials/analysis tools: CCH BA MMT. Wrote the paper: YM CCH MMT SEQ. References 1. Belteki G, Haigh J, Kabacs N, Haigh K, Sison K, et al. (2005) Conditional and inducible transgene expression in mice through the combinatorial use of Cre- mediated recombination and tetracycline induction. Nucleic Acids Res 33: e51. 2. Maezawa Y, Kreidberg JA, Quaggin SE (2012) CHAPTER 1- Embryology of the Kidney. Brenner and Rector’s the Kidney. Philadelphia, PA: W.B. Saunders Company. Figure 5. Ptch1 fl/fl ;Tcf21-Cre kidneys develop multiple renal cysts. (A) Control kidney (left) and Ptch1 fl/fl ;Tcf21-Cre kidney (right). (B) Histology of control kidney. (C) Multiple cysts of Ptch1 fl/fl ;Tcf21-Cre kidney. (D) Glomerular cysts as well as tubular cysts were observed in the mutant kidney. (E, F) Kidneys were stained with LTL-FITC (green) and THP (red) that mark proximal tubules or loop of Henle, respectively. (E) Control kidney shows beautiful organized structure of proximal tubules and loops of Henle. (F) Disorganized structure of proximal tubule and loop of Henle in Ptch1 fl/fl ;Tcf21- Cre kidney. Loops of Henle are very short. Some cysts were stained with either LTL or THP. (G–J) Sections were stained with DBA, which marks collecting ducts. (G, I) Control kidney show organized structure of collecting duct. Note the straight configuration of cortical collecting duct. (H, J) Structure of collecting duct system is severely disorganized in Ptch1 fl/fl ;Tcf21-Cre kidney. The path of collecting duct is random and winding. (K, L) Sections were stained with LTL (green) and Ki67 for proliferation. (K) normal kidney (L) Ptch1 fl/fl ;Tcf21-Cre kidney. Note that Ki67 is frequently positive in the cystic wall. (M and N) In Situ hybridization for Gli1 , an established downstream target of Shh pathway. (M) Control kidney shows activation of Shh pathway in the interstitial area (N) Mutant kidney shows increased Gli1 expression in condensing mesenchyme (black arrowhead) and stroma, but is negative in pretubular aggregates (black arrow) and nephrogenic vesicles (white arrowhead). Magnification: B, C 40 6 , D, G, H 100 6 , E, F, I–N 200 6 . doi:10.1371/journal.pone.0040547.g005 Generation of Tcf21/Pod1-Cre Mouse PLoS ONE | 7 July 2012 | Volume 7 | Issue 7 | e40547
Image of page 7
3. Dressler GR (2009) Advances in early kidney specification, development and patterning. Development 136: 3863–3874. 4. Kobayashi A, Valerius MT, Mugford JW, Carroll TJ, Self M, et al. (2008) Six2 defines and regulates a multipotent self-renewing nephron progenitor population throughout mammalian kidney development. Cell Stem Cell 3: 169–181. 5. Srinivas S, Goldberg MR, Watanabe T, D’Agati V, al-Awqati Q, et al. (1999)
Image of page 8

You've reached the end of your free preview.

Want to read all 8 pages?

  • Fall '20
  • john
  • The Land, Nephron, PLOS One, mesenchyme, hydroureter

What students are saying

  • Left Quote Icon

    As a current student on this bumpy collegiate pathway, I stumbled upon Course Hero, where I can find study resources for nearly all my courses, get online help from tutors 24/7, and even share my old projects, papers, and lecture notes with other students.

    Student Picture

    Kiran Temple University Fox School of Business ‘17, Course Hero Intern

  • Left Quote Icon

    I cannot even describe how much Course Hero helped me this summer. It’s truly become something I can always rely on and help me. In the end, I was not only able to survive summer classes, but I was able to thrive thanks to Course Hero.

    Student Picture

    Dana University of Pennsylvania ‘17, Course Hero Intern

  • Left Quote Icon

    The ability to access any university’s resources through Course Hero proved invaluable in my case. I was behind on Tulane coursework and actually used UCLA’s materials to help me move forward and get everything together on time.

    Student Picture

    Jill Tulane University ‘16, Course Hero Intern

Stuck? We have tutors online 24/7 who can help you get unstuck.
A+ icon
Ask Expert Tutors You can ask You can ask You can ask (will expire )
Answers in as fast as 15 minutes
A+ icon
Ask Expert Tutors