Fiehn BIS103 lecture 17_fall 2010

Quantitative analytical biochemistry accuracy and

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Quantitative analytical biochemistry: “accuracy” and “precision” Low accuracy, low precision Low accuracy, high precision High accuracy, low precision High accuracy, high precision
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8 Application ‚metabolite profiling‘ fasted and fed mice (liver) 386 392 rt intens.3 E^04 intens. 5 E^05 752 758 rt glucose m/z 319 β -hydroxybutyrate m/z 233 intens. 8 E^06 400 800 retention time (s) 1200 intens.4 E^03 797 rt galactonate m/z 333 intens.1.4 E^04 827 rt inositol m/z 318 intens.1.4 E^04 915 rt 926 mannose-6P m/z 387 glucose-6P (1) m/z 387 glucose-6P (2) m/z 387 galactose-6P m/z 387 Example of metabolite profiling of 3 mg FW of mouse liver tissues for each 3 female BI6 mice, fed (blue) and fasted (red). Total ion chromatogram from GC-TOF analyses (lower panel) are magnified for peaks corresponding to abundant ß-hydroxybutyrate, malate, aspartate, glucose, galactonate, inositol and hexose phosphates (left-to-right labeled peaks, respectively). Raw data are analyzed and annotated using a custom specialized database (BinBase). intens. 7 E^04 563 rt 580 malate m/z 233 aspartate m/z 232
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