1984 lucasfilm ltd lab 6 page 10 6 use a small

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© 1984 LucasFilm, Ltd.
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Lab 6 Page 10 6. Use a small Pipette Tip and your P200 Microtiter Pipette to add 100 μ l (0.1 ml) of your Water Sample to each of your 3 “100” Tubes of LSL Broth. • Your P200 Microtiter Pipette should be set at 1 | 0 | 0 for 100 μ l. - Gently Finger Vortex each Tube. 7. Place your Tubes back in the Plastic Container and put it in the Boarding Zone for 37°C for 24 Hours 8. Place your Flask in the Stainless Steel Bus Pan. • Remove the Foil Cap and give it to Oscar Please do not pour your Water Sample down the Sink. _______________________________ “And now, you give me the diamond.” ‘Are you trying to develop a sense of humor, or am I going deaf?’ What’s that? Antidote.” To what?’ The poison you just drank.” © 1984 LucasFilm, Ltd.
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Lab 6 Page 11 Bacteriophage Titer Results 1 Retrieve your Bacteriophage Agar Overlays. Arrange them as below: Label: 4 5 6 7 8 9 Dilution: 10 -4 10 -5 10 -6 10 -7 10 -8 10 -9 2. Your 4, 5 and possibly your 6 Plate (10 -4 , 10 -5 , and = 10 -6 Plates) will probably be uncountable. The Plaques will have flowed together and become a Confluent Plaque (L= flow together). 3 Your 7, 8 and maybe even your 9 Plate (10 -7 , 10 -8 and 10 -9 Plates) should have usable Numbers of Plaques, although you may want to Quadrant the 7 Plate. It’s likely that the 9 Plate will have too few Plaques to be used for statistically Significant Calculations. 4. Count the Plaques on each Plate with a Decent Number of Plaques. The Clickers help a whole lot. 5. To determine the Number of Pfu’s in the Phage Suspension, divide the Number of Plaques by the Dilution Factor (which mean merely make the Dilution Factor Positive). • So if you have, say, 123 Plaques on your 7 Plate this becomes 123 x 10 7 Plaques. • Convert this to Scientific Notation and you’ve got 1.23 x 10 9 • Add the appropriate Units and it’s 1.23 x 10 9 Pfu/ml. • Everyone in the same Lab used the same Phage Suspension so everyone’s Numbers should work out to a similar Burst Size. ________________________ Wow! Holy Smoke! Crash landing! “Short Round, step on it.” Okey dokey, Dr. Jones. Hold on to your potatoes! ‘For crying out loud, there's a kid driving the car!’ “Where's the antidote?” ‘Oh, listen, I just met you! Oh, I'm not that kind of girl...’ Hey, Dr. Jones, no time for love. We've got company. © 1984 LucasFilm, Ltd.
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Lab 6 Page 12 Gram Positive Biochemical Tests Catalase Materials (per Clan): • Sterile Toothpicks • Microscope Slide • Hydrogen Peroxide (Clear Stuff in an Ice Bath on the Back Bench) • Our Streptococcus lactis Negative Control • Your Skin Isolate (Micrococcus luteus or Staphylococcus epidermidis ). The Catalase Test only makes Sense when you perform it on Gram Positive Cocci. Procedure 1. Use a Sterile Toothpick to transfer a Goober of your Patient Skin Isolate (Micrococcus luteus or Staphylococcus epidermidis ) to the Left Side of a Slide.
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