Peroxide which when combined with peroxidase phenol

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peroxide which when combined with peroxidase, phenol and 4-aminoantpyrine to yield quinoneimine dye. Principles: When cholesterol esters react with cholesterol esterase, they are hydrolyzed to produce cholesterol and fatty acids, the cholesterol is then oxidized by cholesterol oxidase to form hydrogen peroxide and cholesterol-3-one, the hydrogen peroxide and phenol and 4- aminoantipyrine are catalyzed by peroxidase to form water and quinoneimine dye which changes the sample color to a red dye. The intensity of the red color produced is proportional to the amount of cholesterol present in the sample. Cholesterol Esters --------C. Esterase----- > Cholesterol + Fatty Acids Cholesterol + O ---------C. Oxidase ------> Cholesten-3-one + H2O2 2 H O + 4-AAP + Phenol -------Peroxidase------ > Quinoneimine (red dye) + 4 H O Materials and Methods: The equipment used during the laboratory experiment include a Spectrophotometer, pipettes, automatic pipettes, disposable pipette tips, cuvettes, test tube racks, and timers. The reagents, QCs, and standard used in this experiment include the Patient Level 1- Unassayed Chemistry Control (Human) which expired on June 30, 2010 and had the lot number 16411, the Cholesterol Reagent which expired in August of 2011 and had a lot number of 005501-190, the Standard which expired in November 2011 and had a lot number of 814403-211, Quality Control 1 which expired on Mart 31, 2011 and had a lot number of CHA 11031, and Quality Control 3 which expired on March 31, 2011 and had a lot number of CHA 11033.
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