5.Repeat steps 2-4 with the 40x power objective lens. 6.When you have finished your observations of this slide (or any slide), rotate the nosepiece until the lowest power objective clicks into place, and then remove the slide. Diameter of Field (Field of view)The diameter of the field(the circle visible through the lens) is the length of the field across the center. View a slide under the microscope on both the scanning and a higher power objective. Which objective gives the least and the greatest area of view?____________________________________________________________________________IV. Using a Dissecting Microscope.1.Dissecting microscopes allow you to see a 3 – dimensional image of an object.2.Look at the bee wing and flea at .
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Conclusion Questions:1. State 2 procedures which should be used to properly handle a light microscope.2. Explain why the light microscope is also called the compound microscope.3. Images observed under the light microscope are reversed and inverted. Explain what this means.4. Explain why the specimen must be centered in the field of view on low power before going to high power.5. A microscope has a 20 X ocular (eyepiece) and two objectives of 10 X and 43 X respectively:a.) Calculate the low power magnification of this microscope. Show your formula and all work.b.) Calculate the high power magnification of this microscope. Show your formula and all work.6. In three steps using complete sentences, describe how to make a proper wet mount of the letter e.7. Describe the changes in the field of view and the amount of available light when going from low to high power using the compound microscope.8. Explain what the microscope user may have to do to combat the problems incurred in question #7.9. How does the procedure for using the microscope differ under high
power as opposed to low power?10. Indicate and describe a major way the stereomicroscope differs from the compound light microscope in terms of its use.11. What is the advantage of using a dissecting microscope?12. Does low power or high power have a larger field of view and allow you to see more of the object?13. Which has a smaller field but magnifies to a greater extent?14. Why is it helpful for a microscope to be parfocal? 15. What is inversion? 16. Why is locating an object more difficult if you start with the higher powerobjectives than with the scanning objective?
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