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Psy137BasicsandMethods

Immunocytochemistry identifying neurons that contain

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Immunocytochemistry Identifying neurons that contain a particular type of receptor Immunocytochemistry Localization of the receptor Ex. Can stain for an estrogen receptor Autoradiography Localization of a radioactive ligand Ex. Can stain neurons that have a particular receptor subtype
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Immunocytochemistry Identifying neurons that produce a particular neurotransmitter Immunocytochemistry Localization of a peptide or protein Ex. Stain for an AChE Localization of synthesizing enzyme Ex. Stain for tyrosine hydroxylase
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Blot Tests A cellular technique that is used to discern if a particular protein (or nucleic acid is found in a given tissue. The various forms of these techniques involve electrophoresis of homogenates of tissues from which proteins are extracted. Electrophoresis will separate these proteins on the basis of electrical charge.
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Stimulation &/or Recordings Direct electrical stimulation of neuronal pools (brain, spinal cord, or peripheral nerves) can elicit electrical activity in cells and tissues of neural relevant sites. Can stimulate and then record Or just record during specific behaviors
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Pharmacological Techniques The use of chemical agents that may alter the action or function of a hormone. The two major groups are referred to as: AGONISTS – hormone mimics ANTAGONISTS – hormone blockers These compounds can be administered to an animal in a variety of ways but the two most common forms are: Cannulation: chronic indwelling Single injection: using a syringe and needle
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Brain Imaging Computerized tomography (CT)- the use of device that employs a computer to analyze data obtained by a scanning beam of X-rays to produce a 2-D picture
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