1986 compared the API 20E rapid identification system for Aer hydrophila with

1986 compared the api 20e rapid identification system

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(1986) compared the API 20E rapid identification system for Aer. hydrophila with Kaper's medium (Kaper et al, 1979) and conventional biochemical tests. Kaper and co-workers formulated a single-tube medium, which was suitable for determining motility, inositol and mannitol fermentation, ornithine decarboxylase and deamina- tion, and the production of H2S and indole. Thus, bona fide isolates of Aer. hydrophila gave an alkaline reaction on the top of the medium, acid production in the butt, motility, and indole, but not H2S production (H2S production may occur on the top). Toranzo and colleagues pointed to shortcomings of the API 20E system, insofar as many environmental isolates were mis-identified or not Hsted by the published profile index. In contrast, Kaper's medium was effective for fast, presumptive identification. Problems were encountered with the reliability of some conventional biochemical tests, notably the Voges Proskauer reaction, fermentation and gas production from arabinose, gelatinase production, and the lysine decarboxylase test. Ironically, these tests have also been considered to be correlated with virulence in motile aeromonads. Nevertheless, it is accepted that the API 20E and API 20NE systems have a role in the diagnosis of bacterial fish pathogens. Consequently, diagnostic schemes based on these systems are included in Tables 6.3 and 6.4. In addition, API-zym is very useful for diagnosing Ren. salmoninarum (See Table 6.5), which gives a characteristic profile: - + - + - + + - + + + +/ A diagnostic scheme for use with Biolog-GN has been included as Table 6.6. Diagnoses may also be achieved by means of diagnostic schemes based on reactions in conventional phenotypic tests. The procedure may be automated, as with the Abbott Quantum II system (Teska et al, 1989), and involves spectrophoto- metric readings at 492.6 nm, with a sample cartridge containing 20 inoculated biochemical chambers.. Alternatively, specially constructed diagnostic tables may be used, such as presented in Tables 6.7 and 6.8. Diagnosis of botuHsm has been accomplished by isolation of CI. botulinum from diseased tissues and, more importantly, by demonstrating the presence of circulating toxin (particularly to CI. botulinum type E) in the blood of moribund fish (Cann and Taylor, 1982). Presumptive identification of Lactococcus garvieae-likc organisms has been made following growth on bile (40%)-aescuHn agar (see Facklam and Moody, 1970), with hydrolysis of aesculin and by the characteristic growth on eosin-methylene blue agar (lactose is not fermented). Bacillus mycoides could be distinguished from other bacilli, as follows (Goodwin et al, (1994): Presence of Rhizoidal Motility Growth parasporal crystals growth at 45°C Bacillus mycoides + Bacillus anthracis + Bacillus cereus + + Bacillus thuringiensis -\- + +
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  • Bacteria, representative, gram-negative bacteria

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