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cigarette smoke will fill the container, but will be allowed to settle. After smoke has settled, move flies from vial three into vial four exposing them to the smoke residue. After two weeks, observe the flies under a microscope and take note of any new or notable phenotypic genetic mutations. For example, change in eye color and short or curly wings. Keep track numerically of the amount of phenotypic mutations in each vial. Statistical Analysis: This experiment will require a paired t-test. A paired t-test will be used because population standard deviation is not known and we are testing the mean difference. A paired t-test determines if the mean difference between the observed sets of data deviates significantly from zero. A positive mean difference suggests third-hand smoke causes more mutations than second-hand smoke, a negative mean difference suggests second-hand causes more phenotypic mutations than third-hand, and a difference of zero suggests no difference. Proposed Point Value: I propose that this proposal is worth 125 points because it could lead to the discovery of new direct and indirect health risks linked to smoking tobacco.
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