Principles when creatinine reacts with sodium picrate

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Principles: When creatinine reacts with sodium picrate (picric acid) in alkaline conditions, creatinine-picrate complex in formed, a color complex, which absorbs at 510 nm. The rate of formation of color is proportional to the creatinine in the sample. Creatinine + Sodium Picrate ---------Alkali------> Creatinine-picrate complex (yellow-orange) Materials and Methods: The equipment used during the laboratory experiment include a Spectrophotometer, pipettes, automatic pipettes, disposable pipette tips, cuvettes, test tube racks, heat blocks, and timers. The reagents, QCs, and standard used in this experiment include the Patient Level 1- Unassayed Chemistry Control (Human) which expired on June 30, 2010 and had the lot number 16411, the Creatinine Reagent –R1 which expired in November of 2011 and had a lot number of 932101- 200, Creatinine Reagent –R2 which expired in November of 2011 and had a lot number of 932101-200, the Standard (2.5 mg/dl) which expired in March 2010 and had a lot number of 807201-224, Quality Control 1 which expired on March 31, 2011 and had a lot number of CHA 11031, and Quality Control 3 which expired on March 31, 2011 and had a lot number of CHA 11033. Results: Sample Absorbance 1 at 510 nm Absorbance 2 at 510 nm Blank 0.00 0.00 Patient 0.320 0.100 Standard 0.060 0.107 QC Level 1 0.063 0.075 QC Level 3 0.212 0.395 Calculations:
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Patterson Blank: 0.00/0.047 x 2.5 mg/dl = 0.00 mg/dl Patient: 0.068/0.047 x 2.5 mg/dl = 3.617 mg/dl Standard: 0.047/0.047 x 2.5 mg/dl = 2.5 mg/dl QC Level 1: 0.012/0.047 x 2.5 mg/dl = 0.638 mg/dl QC Level 3: 0.183/0.047 x 2.5 mg/dl = 9.734 mg/dl Discussion:
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