Have a characteristic flat spreading matte

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have a characteristic flat, spreading “Matte Construction Paper” or “Frosted Glass” Appearance. This tells you (A) an individual Bacillus must look a lot like that Hot Dog that got left of the Grill overnight, and (B) Motile Bacteria wriggle away from each other and spread-out into what’s frequently a Monolayer. Bacillus cereus is on our all-singing, all-dancing Nutrient Agar. The Colonies are large and flat (because the Bacteria are Motile) and the Colonies have a Matte Construction Paper Appearance due to the wrinkled Shape of each individual Bacterium. Since Bacillus is a Spore-former, and since Spores are highly resistant to Heat, a simple Pasteurization Step can be used to further increase the relative Representation of Bacillus in a Soil Sample. Most Soil Bacteria cannot survive heating to 80°C. Their Proteins are denatured at this Temperature. But the Proteins in Bacterial Spores do not denature at this Temperature (probably due to the near Total Elimination of Water Content during Spore Formation). There are two commonly encountered Spore-Forming Bacteria in Soil Samples: Bacillus and Clostridium . The Genus Clostridium includes the Causative Agents of Botulism, Gangrene, and Tetanus. ¡Canasta! We don’t want to mess with any of those Diseases! But Clostridium Species are Obligate Anaerobes (they cannot tolerate Oxygen). Bacillus can be separated from Clostridium by Incubation under Aerobic Conditions. Bacillus is the only Spore-Forming Species that can grow under our Aerobic Incubation Conditions. These two Traits -- the Ability to produce Heat-Resistant Spores and the Ability to grow Aerobically -- provide the Basis of our Bacillus Selection Strategy. A Soil Sample will be suspended in Water and Pasteurized to kill all Vegetative Bacteria. This Pasteurized Suspension -- containing only Spores -- is streaked onto a Nutrient Agar Plate, which will then be incubated Aerobically. Bacillus Spores and the Clostridium Spores will both germinate but only the Vegetative Cells of Bacillus will be able to grow under these Conditions. _________________________________________________________________ “Rest easy, son. You've had a busy day. You're fortunate to be all in one piece.” Ben? Ben Kenobi? Boy, am I glad to see you.’ The Jundland Wastes are not to be traveled lightly. Tell me, young Luke, what brings you out this far?” This little droid. I think he's searching for his former master. I've never seen such devotion in a droid before. Ah, he claims to be the property of an Obi-Wan Kenobi. Is he a relative of yours? Do you know what he's talking about?’ Obi-Wan Kenobi. Obi-Wan... Now, that's a name I've not heard in a long time. A long time.” © 1977 Lucasfilm, Ltd.
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Lab 1 Page 14 Bacteria found in Food Humans have been domesticating Bacteria for Millennia (L= thousands [of years]) for the Production of Food. Buttermilk, Yogurt and Cheese are simply Milk inoculated with Bacteria that ferment the Carbohydrates and change the Consistency and Flavor. Sauerkraut and Kimchi are Cabbage inoculated with Bacteria that ferment Carbohydrates.
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