bch370_sp19m_class_3_questions_sol.pdf

See the class slides the key point is that in

This preview shows page 3 - 4 out of 4 pages.

See the class slides.  The key point is that in fluorescence, the excitation wavelength is  different from the emission wavelength, so there are no background photons at the  fluorescence wavelength. 4)  Tryptophan is a good fluorophore. 5)  (a) Three coenzymes that are good fluorophores: NADH, FAD, pyridoxyl phosphate (PLP). (b) NADH is a hydride donor, used in gluconeogensis (and many other pathways). FAD is an electron carrier found within the pyruvate dehydrogenase complex (PDC), and many  other places. PLP is found in the active site of transaminase enzymes (and many other places).
Image of page 3

Subscribe to view the full document.

6) (a)  Fluorescence quenching is the observation that the presence of some molecules  (fluorescence quenchers) can reduce the probability of fluorescence occuring, by providing an  alternate (non-radiative) pathway for relaxation to the electronic ground state. (b)  Tryptophan fluorescence versus (iodide) quencher concentration, for a protein that contains a  single  tryptophan that is on the protein’s surface : The plot would most likely have a negative slope (such as shown below). (c)  For tryptophan fluorescence versus (iodide) quencher concentration, for a protein that  contains a single tryptophan that is buried in the protein’s hydrophobic core: The plot would have a near-zero or slightly negative slope. For example, something like this:
Image of page 4
You've reached the end of this preview.
  • Spring '16
  • HOFFMAN
  • 100 mm, PLP, Hoff, 50 degrees, 5 mm

{[ snackBarMessage ]}

What students are saying

  • Left Quote Icon

    As a current student on this bumpy collegiate pathway, I stumbled upon Course Hero, where I can find study resources for nearly all my courses, get online help from tutors 24/7, and even share my old projects, papers, and lecture notes with other students.

    Student Picture

    Kiran Temple University Fox School of Business ‘17, Course Hero Intern

  • Left Quote Icon

    I cannot even describe how much Course Hero helped me this summer. It’s truly become something I can always rely on and help me. In the end, I was not only able to survive summer classes, but I was able to thrive thanks to Course Hero.

    Student Picture

    Dana University of Pennsylvania ‘17, Course Hero Intern

  • Left Quote Icon

    The ability to access any university’s resources through Course Hero proved invaluable in my case. I was behind on Tulane coursework and actually used UCLA’s materials to help me move forward and get everything together on time.

    Student Picture

    Jill Tulane University ‘16, Course Hero Intern