RNAs and their role in protein synthesis; RNA polymerase and transcription; regulation of transcription in prokaryotes; post transcriptional processing; the genetic code; structure of transfer RNA; ribosome structure; translational process, protein synthesis inhibitors; control of eukaryotic translation; post translational modification; protein degradation; nonribosomal polypeptide synthesis; structure and genomic organization; regulation of eukaryotic gene expression; cell differentiation; oncogenes and cancer.
37 Recombinant DNA Technology 2 Credits DNA cloning methods; cloning vectors including plasmids, bacteriophages, cosmids, YAC vectors, shuttle and expression vectors; gene splicing, genomic libraries, screening methods for gene libraries; DNA cloning restriction enzymes; DNA lijase; tumor inducing (Ti) plasmids; Southern and Northern blotting; chromosome walking; PCR; site specific mutagenesis; production of proteins. Potentials of recombinant DNA technology; gene therapies; restriction fragment length polymorphisms and disease detection (e.g. cystic fibrosis); human genome project; social considerations. Microbial Biochemistry and Genetics 2 Credits Classification and characteristics of main groups of microorganisms; light microscopy and microbial identification; biophysical and biochemical factors for growth, culture enrichment and isolation; anaerobic cultures; solid cultures; batch and continuous cultures; methods of growth measurement; culture preservation; mutation and protoplast fusion for culture improvement; gene transfer by transformation, transduction and conjugation; microbial metabolism; growth under extreme environments; archaebacteria; structure and biology of tobacco mosaic virus; spherical viruses; bacteriophages; influenza virus; cell immobilization. Computers and Skills for Biochemists 2 Credits Computers for biochemists; basic principles; spreadsheet work; word processing; graphical and statistical packages; document preparation; software for biochemistry teaching and research; experimental design. Textbooks, scientific literature, data presentation, report writing, etc. Lab. III 2 Credits Cultivation of microorganisms in the laboratory using solid and liquid culture media; test tube, Petri plate and shake flask cultures; isolation of pure culture; bacterial growth curve; preservations of microbial strains. Preparation and estimation of chromosomal and plasmid DNA and RNA.
38 Lab. IV 2 Credits A miniproject to clone selected genes in E. coli; praparation of chromosomal DNA; minipreparation of plasmid DNA; restriction of chromosomal and plasmid DNA and fractionation of DNA by agarose gel electrophoresis; electroelution of selected DNA fragments; alkaline phosphatase treatment of linearised plasmid DNA; ligation to produce recombinant DNA; transfomation of E. coli by competent cell and electroporation methods; screening for blue and white colonies on X-gal plates; detection of transformants carrying the cloned gene. Isolation of the recombinant DNA, its subcloning and development of restriction mapping.
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