RNAs and their role in protein synthesis RNA polymerase and transcription

Rnas and their role in protein synthesis rna

This preview shows page 36 - 39 out of 43 pages.

RNAs and their role in protein synthesis; RNA polymerase and transcription; regulation of transcription in prokaryotes; post transcriptional processing; the genetic code; structure of transfer RNA; ribosome structure; translational process, protein synthesis inhibitors; control of eukaryotic translation; post translational modification; protein degradation; nonribosomal polypeptide synthesis; structure and genomic organization; regulation of eukaryotic gene expression; cell differentiation; oncogenes and cancer.
Image of page 36
37 Recombinant DNA Technology 2 Credits DNA cloning methods; cloning vectors including plasmids, bacteriophages, cosmids, YAC vectors, shuttle and expression vectors; gene splicing, genomic libraries, screening methods for gene libraries; DNA cloning restriction enzymes; DNA lijase; tumor inducing (Ti) plasmids; Southern and Northern blotting; chromosome walking; PCR; site specific mutagenesis; production of proteins. Potentials of recombinant DNA technology; gene therapies; restriction fragment length polymorphisms and disease detection (e.g. cystic fibrosis); human genome project; social considerations. Microbial Biochemistry and Genetics 2 Credits Classification and characteristics of main groups of microorganisms; light microscopy and microbial identification; biophysical and biochemical factors for growth, culture enrichment and isolation; anaerobic cultures; solid cultures; batch and continuous cultures; methods of growth measurement; culture preservation; mutation and protoplast fusion for culture improvement; gene transfer by transformation, transduction and conjugation; microbial metabolism; growth under extreme environments; archaebacteria; structure and biology of tobacco mosaic virus; spherical viruses; bacteriophages; influenza virus; cell immobilization. Computers and Skills for Biochemists 2 Credits Computers for biochemists; basic principles; spreadsheet work; word processing; graphical and statistical packages; document preparation; software for biochemistry teaching and research; experimental design. Textbooks, scientific literature, data presentation, report writing, etc. Lab. III 2 Credits Cultivation of microorganisms in the laboratory using solid and liquid culture media; test tube, Petri plate and shake flask cultures; isolation of pure culture; bacterial growth curve; preservations of microbial strains. Preparation and estimation of chromosomal and plasmid DNA and RNA.
Image of page 37
38 Lab. IV 2 Credits A miniproject to clone selected genes in E. coli; praparation of chromosomal DNA; minipreparation of plasmid DNA; restriction of chromosomal and plasmid DNA and fractionation of DNA by agarose gel electrophoresis; electroelution of selected DNA fragments; alkaline phosphatase treatment of linearised plasmid DNA; ligation to produce recombinant DNA; transfomation of E. coli by competent cell and electroporation methods; screening for blue and white colonies on X-gal plates; detection of transformants carrying the cloned gene. Isolation of the recombinant DNA, its subcloning and development of restriction mapping.
Image of page 38
Image of page 39

You've reached the end of your free preview.

Want to read all 43 pages?

  • Left Quote Icon

    Student Picture

  • Left Quote Icon

    Student Picture

  • Left Quote Icon

    Student Picture