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This was due to the separation of the mixed culture

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due to the separation of the mixed culture into a pure one. The agar was separated into four different quadrants, the pure cultures can be seen in the third and fourth quadrants.10
Experiment 1.6 In experiment 1.6, the pure cultures formed in experiment 1.5, was used to streak plate the E. coliand S. aureus into two new agar plates.Table G: Growth of E. coliand S. aureus on the agar plate Physical CharacteristicsE. coliS. aureusConfigurationRoundRound ElevationFlat RaisedMarginsEntirewavyColourBeige WhiteClarityTranslucent OpaqueSizeLarge Smaller than E.coliTable G summarizes the observation that was seen on the agar plate after incubation. In the agar plate that was streaked with E.coli, the colonies included a round configuration, flat elevation, entire margin, and was beige and translucent and was large. In the agar plate that was streaked with S. aureus, the colonies included a round configuration, raised elevation, wavy margin, and was white and opaque, and colonies smaller that E.coliExperiment 1.7 In experiment 1.7, a dilution series was performed in preparation for experiment 1.8 and experiment 1.9. The dilution series is performed to lower the concentration of bacterial cells in order to achieve well-isolated colonies. The sample is diluted using proper aseptic techniques and a sterile pipette, to mix a bacterial culture into a known volume of known of sterile broth buffer which acts as the dilute. The test tubes were labelled 1 to 5 accordingly. This experiment used E.coli.Table H: Concentration of E.coli in each test tube 11
Test tube 1 Test tube 2 Test tube 3Test tube 4 Test tube 5 Concentration1volumeof bacterialcultureused110=10110.1=10210.01=10310.001=1010.0001=10Table H above shows the calculation of the concentration of E.coli in each test tube, which was calculated by one divided by the volume of bacterial cells used.Experiment 1.8 This experiment included the used of the spread plate technique, which was used to obtain isolated colonies for recording purposes. This experiment used the diluted test tube 4 and 5 from experiment 1.7, which is placed in the center of an agar plate and spread evenly using a glass spreader, the purpose of this is to allow the growth of bacteria on the surface of the agar. The results of the dilution from experiment 1.7, was the presence of well-isolated colonies of E.coli. The first agar plate that used test tube four from experiment 1.7 includes 188 colonies that come in various sizes and exhibit a flat configuration. The dilution factor of 104and calculated a total of 1.9107(CFU/mL). The second agar plate, however, had fewer colonies compared to the first one. There was a total of 39 colonies in this agar plate which calculated to atotal of 3.9107CFU/mL since the dilution factor was 105. As a result, the average number of cells in the original sample is calculated as 2.9107CFU/mLCFUmL=¿of coloniesdilution factorvolumeincoculated(mL)Tube 4:CFUmL=1881040.1CFUmL=1.9107Tube 5 12
CFUmL=391050.1CFU

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Term
Fall
Professor
NoProfessor
Tags
Bacteria, Agar plate

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