Note Jan 28, 2013 lecture 1

Can take two genetically identical mice and treat one

Info icon This preview shows pages 1–2. Sign up to view the full content.

View Full Document Right Arrow Icon
Can take two genetically identical mice and treat one but not the other and then compare( how does the treatment affect the organism differently?) Isolate cells to grow in the lab- cell culture Can choose the different type of tissue you like-use what tissue is relevant to your work or what's easy to use fibroblasts- dermis "connective tissue" - relatively easy to grow in culture Epithelial cells-line our insides, cost organs Isolate cells from an organism: usually dissect them out of the tissue Disconnect from matrix and other cells Proteases cut protein linking cells Calcium- connects cells to one another- EDTA/EGTA breaks these connections Add culture medium What do cells need to grow? Sugar(glucose), salts, AA, vitamins (cofactors for enzymes), serum (liquid portion of blood)- contains some nutrients and growth factors, tells cells to grow and divide Keep human cells at body temp -37C Primary culture cells- grow connecting to the bottom of a T-100/25 Passaging cells (passing) which creates a secondary culture- lasts days/weeks Cells will sines and stop dividing Limited to the amount of cells you can cultures
Image of page 1

Info iconThis preview has intentionally blurred sections. Sign up to view the full version.

View Full Document Right Arrow Icon
Immortalized cells lines Will grow and divide indefinitely (much longer time)- ignore signals that tell them to stop growing Up regulate telomerase- express telomerase and get an extension of the sequence at the ends of chromosomes Transformed cell lines Have a cancerous phenotype Can induce a cell to become cancerous Divide without regard to the cell cycle
Image of page 2
This is the end of the preview. Sign up to access the rest of the document.

{[ snackBarMessage ]}

What students are saying

  • Left Quote Icon

    As a current student on this bumpy collegiate pathway, I stumbled upon Course Hero, where I can find study resources for nearly all my courses, get online help from tutors 24/7, and even share my old projects, papers, and lecture notes with other students.

    Student Picture

    Kiran Temple University Fox School of Business ‘17, Course Hero Intern

  • Left Quote Icon

    I cannot even describe how much Course Hero helped me this summer. It’s truly become something I can always rely on and help me. In the end, I was not only able to survive summer classes, but I was able to thrive thanks to Course Hero.

    Student Picture

    Dana University of Pennsylvania ‘17, Course Hero Intern

  • Left Quote Icon

    The ability to access any university’s resources through Course Hero proved invaluable in my case. I was behind on Tulane coursework and actually used UCLA’s materials to help me move forward and get everything together on time.

    Student Picture

    Jill Tulane University ‘16, Course Hero Intern