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Note Jan 28, 2013 lecture 1

Isolate cells to grow in the lab cell culture can

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Isolate cells to grow in the lab- cell culture Can choose the different type of tissue you like-use what tissue is relevant to your work or what's easy to use ±broblasts- dermis "connective tissue" - relatively easy to grow in culture Epithelial cells-line our insides, cost organs Isolate cells from an organism: usually dissect them out of the tissue Disconnect from matrix and other cells Proteases cut protein linking cells Calcium- connects cells to one another- EDTA/EGTA breaks these connections Add culture medium What do cells need to grow? Sugar(glucose), salts, AA, vitamins (cofactors for enzymes), serum (liquid portion of blood)- contains some nutrients and growth factors, tells cells to grow and divide Keep human cells at body temp -37C Primary culture cells- grow connecting to the bottom of a T-100/25 Passaging cells (passing) which creates a secondary culture- lasts days/weeks Cells will sines and stop dividing Limited to the amount of cells you can cultures
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Immortalized cells lines Will grow and divide indefnitely (much longer time)- ignore signals that tell them to stop growing Up regulate telomerase- express telomerase and get an extension oF the sequence at the ends oF chromosomes TransFormed cell lines Have a cancerous phenotype Can induce a cell to become cancerous Divide without regard to the cell cycle
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Isolate cells to grow in the lab cell culture Can choose...

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