Lucci_Prelab6_Monday

# What is the objective of this exercise to determine

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Exercise 6.3: 10. What is the objective of this exercise? To determine the effect of substrate concentration on enzyme activity. 11. List the different concentrations of substrate to be used in this exercise. 0%, 0.1%, 0.2%, 0.5%, 0.8%, 1%, 5%, 10% 12. List the enzyme concentration to be used. The undiluted 100 units/mL 40mL enzyme solution from exercise 6.2 13. The graph on page 6-7 is a semi-log graph. What is a semi-log graph? A way of visualizing data that is changing with an exponential relationship; it brings out features in the data that would not easily be seen if both variables had been plotted linearly. This exercise uses a lin-log graph where the x-axis is plotted on a logarithmic scale and the y-axis is plotted on a linear scale. Exercise 6.4: 14. What is the objective of this exercise? To determine the effect of temperature on enzyme activity. 15. Identify the following: i) temperatures to be tested. 4 ° C, 15 ° C, 23 ° C, 37 ° C, boiled ii) Enzyme concentration to be used. The undiluted 100 units/mL enzyme solution from exercise 6.1 iii) substrate concentration to be used. 40 mL 1% H 2 O 2 solution = 0.294M H 2 O 2 iv) independent variable. temperature v) dependent variable. Rate of reaction Exercise 6.5: 16. What is the objective of this exercise? To determine the effect of pH on enzyme activity.

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17. Identify the following: i) pHs to be tested. 4, 6, 7(control), 8, 10 ii) Enzyme concentration to be used.
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