9 Wait 15 minutes 10 Hold the IKI spray cm away from the paper

9 wait 15 minutes 10 hold the iki spray cm away from

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and vegetables, you may want to squeeze or mash it to get some liquid on the paper. 9. Wait 15 minutes. 10. Hold the IKI spray bottle 25 - 30 cm away from the paper towel, and mist with the IKI solution. Do the same with your negative control. 11. Record your results in Table 1. Wait 10 - 15 minutes if the results are not immediately obvious. Observe the color of the starch control, the negative control, and where the saliva and two fruit/vegetable samples were applied. Consider what these results indicate. For the saliva/fruit/vegetable samples, record the color where they were applied not the surrounding area. Is there a difference? Set-up a negative control for this experiment in the “negative control” quadrant. Use your knowledge of the scientific method and experimental controls to establish this component. Identify your negative control in Table 1. Hint : What result would you expect if amylase was not present when tested with IKI solution? ©eScience Labs, 2016
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Table 1: Substance vs. Starch Presence Substance Resulting Color Presence of Starch? Presence of Amylase? Starch Control Dark purple Yes Yes Negative Control: Cellulose Brown No No Fruit/Vegetable 1 potato Dark purple Yes No Fruit/Vegetable 2 apple Dark purple Yes No Saliva: Brown No Yes Post-Lab Questions 1. What were your controls for this experiment? What did they demonstrate? Why was saliva included in this experiment? a. Controls were the liquid starch and the cellulose which one, the starch, was supposed to be positive and the cellulose would be negative. Saliva was included because it contains amylase.
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