Results Protein S rates in the whole body after major injury and catabolism to

Results protein s rates in the whole body after major

This preview shows page 22 - 24 out of 70 pages.

Results: Protein S rates in the whole body after major injury , and catabolism to a greater extent than S § After trauma there’s an in whole body Q which comprises an in BOTH rate of S and C with C going up as a higher % This means that N balance under these circumstances would need to be negative - We can speculate on the reasons people get pneumonia (a secondary complication) in hospitals - Another study used a rat model with an induced femur fracture. They infused the rats with 15 N-Gly, killed the animals and removed all the major organs to measure the incorporation of the label in proteins compared to controls. Protein Syn (%/d) Normal/ Control Trauma Liver 17.3 ± 0.9 20.3 ± 1.6* Lung 8.5 ± 1.6 9.4 ± 1.7 NS Heart 15.6 ± 2.3 23.3 ± 4.1* Kidney 15.5 ± 1.6 17.8 ± 1.9* Muscle 4.5 ± 0.9 5.7 ± 0.9* Results: In all the major organs of the traumatic shock rat model ( e.g.- long bone injury in the rat) given 15 N-labeled-Gly and measuring protein S, S significantly except for lung Lung doesn't its protein synthesis as the other organs of the body do in terms of survival after serious injury
Image of page 22
SFLORES_3/13/2014 23 38. INFLUENCE of IMMOBILIZATION STRESS on Q - Q due to immobilization stress o Long bone injury or burn injury and you measure Q over a period of time o Early study done in humans with immobilized leg after tibia fracture. They were on bed rest for at least 37d. Investigators used constant infusion of 13 C-Leu and did muscle biopsies to measure Q Protein Syn (%/d) Protein Catab (by difference) RNA activity (from muscle biopsy; μ g protein syn’d/ hr/ μ g RNA) RNA Specific Activity ( μ g/mg prot) Control 1.65 1.65 0.27 1.88 Immobilized 1.22* 1.53 NS 0.14* 2.25 NS This is not just due to metabolic response in injury, but also due to immobilization of the limb RNA levels change but DNA levels remain fairly constant Rate of protein S ; as a result you’re in negative protein balance because of the leg. But rate of C not affected § Immobilization stress affects rate of S, but catabolic rate stays the same. Not because you have less RNA (because data shows that RNA is there) you just can t syn proteins o This is why the leg is smaller and weaker when in a cast. o So whatever anabolic factors needed for the regulation of S, are being interfered with - Another study took healthy people and put them on bed rest for 14d; crossover study that gave them the same amount of protein (1g/kg BW) and adequate NRG to maintain weight for a period of time. Then the NRG in the diet was to simulate what would happen after injury. Then did Q by first priming system with 13 C bicarbonate and then constant infusion of 13 C-Leu. Took blood and breath samples b/c it was CO 2 [AJCN 86 p.366; 2007]. o No difference in catabolism o Bed rest led to in protein S Even with enough NRG and enough protein (1g/kg BW) the S rate still fell. And on a hypocaloric it got even worse and S suffered even more § With immobilization and involuntary rest; protein C stays the same but protein S and gets worse if NRG intake is lower 39. PROTEIN MASS IN INDIVIDUAL ORGANS - Another study looked at protein mass of different organs in the body. Low protein low NRG
Image of page 23
Image of page 24

You've reached the end of your free preview.

Want to read all 70 pages?

  • Spring '14
  • Knutson,MitchellD
  • NRG

What students are saying

  • Left Quote Icon

    As a current student on this bumpy collegiate pathway, I stumbled upon Course Hero, where I can find study resources for nearly all my courses, get online help from tutors 24/7, and even share my old projects, papers, and lecture notes with other students.

    Student Picture

    Kiran Temple University Fox School of Business ‘17, Course Hero Intern

  • Left Quote Icon

    I cannot even describe how much Course Hero helped me this summer. It’s truly become something I can always rely on and help me. In the end, I was not only able to survive summer classes, but I was able to thrive thanks to Course Hero.

    Student Picture

    Dana University of Pennsylvania ‘17, Course Hero Intern

  • Left Quote Icon

    The ability to access any university’s resources through Course Hero proved invaluable in my case. I was behind on Tulane coursework and actually used UCLA’s materials to help me move forward and get everything together on time.

    Student Picture

    Jill Tulane University ‘16, Course Hero Intern

Stuck? We have tutors online 24/7 who can help you get unstuck.
A+ icon
Ask Expert Tutors You can ask You can ask You can ask (will expire )
Answers in as fast as 15 minutes
A+ icon
Ask Expert Tutors