Effectively for many reasons the use of iodine was

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effectively for many reasons. The use of iodine was successful in showing amylase activitybecause it showed an observable colour change from yellow to blue-black in the presence ofstarch.The use of auto-dispensers,which were pre-measured reduced possible human errorswhich may occur due to judgement errors, and helped fulfil the aim. This added accuracymeant that it was simple to calculate the amylase activity and supplied accurate results.B)The drops of iodine, however, were not auto-dispensed and estimated as equal by eye,which is subject to human error. This could affect the observable colour differences.Therefore, to avoid inconsistencies and possible confounding variables,which may resultfrom discrepancies regarding the amount of iodine being placed in each well, the use of anauto-dispenser could be used next time. Other errors may include cross-contamination of themixtures. In order to avoid this, all equipment should be washed thoroughly after each type ofseed is tested.You need to answer specifically to the questions you were asked.A valid weakness is that the aim is to interpret the role of amylase in plant development, andwe are using only one species of plant. This is logical, as there is nothing in this experimentto say that the experimental outcomes could necessarily allow us to deduce anything aboutother plant species.A valid strength might be that the experiment is aiming to assess the role of amylase inmetabolism, (which is reported as breaking down starch into maltose); this experimentincludes a ‘check’ that amylase is able to break starch down into Maltose (the Benedict’sreaction) – this is logical as this step confirms that the results we measure are due to theactions of amylase as previously reported.ReferencesErnst, D F (1971) Amylase activity in dormant and germinating seeds of Polygonumpensylvanicum.Retrospective Theses and Dissertations.4535.Good effort!
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