Reproductive cloning remove nucleus and take the rest of the cell egg take the nucleus (containing DNA) cell from the body Clone identical to the individual that gave the nucleus Dolly the sheep
Molecular cloning: Principles gene 1 gene 2 2) Make a new piece of DNA gene 1 gene 2 1) Take DNA out of the nucleus cell 1 cell 2 gene 1 gene 2 3) Put new DNA into a test cell and grow copies gene 1 cell divides Daughter cells contain same DNA: Genes 1 and 2 have been cloned gene 2 insert new DNA
Molecular cloning: Applications Normal mouse embryo gene A missing remove a gene to see if anything works differently Loss of function gene is involved in giving the eye its colour eye Reporter gene add a gene that shows us when another gene is working gene is active in blue areas only Lineage tracing mark a group of cells to see where their daughter cells end up gene is passed on to cells all over the body
Tissue Stem Cell Types and Hierarchies
Tissue stem cells: Principles of renewing tissues Stem cell committed progenitors: - “transient amplifying cells” - multipotent - divide rapidly - no self-renewal stem cell: - self renew - divide rarely - high potency - rare specialized cells: - work - no division
Tissue stem cells: Haematopoietic stem cells (HSCs) HSC committed progenitors neutrophil
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- Spring '19