Lab__2a_Media___Culture_-_Collecting_Bacterial_Samples

A quadrant 1 swab from kitchen counter b quadrant 2

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a. Quadrant 1: Swab from kitchen counter b. Quadrant 2: Swab from kitchen sink c. Quadrant 3: Swab from bathroom counter d. Quadrant 4: Swab from bathroom sink After collecting the samples at home, secure Petri dishes shut, and place on top of your refrigerator until you bring them into lab next week. Don’t forget! Make sure that you use rubber bands to secure the Petri dishes that you will be taking home! Images: Bathroom , Libby A. Baker, Kitchen , Gnangarra From the Virtual Microbiology Classroom on ScienceProfOnline.com
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Normal Flora Samples 1. Arm Plate will demonstrate the microorganism inhabiting the surface of the skin. 2. Touch Plates will demonstrate the bacteria in the epidermis of our hands. 3. Throat Swab on Blood agar. 4. Nasal Swabs on TSY & MSA . REMEMBER… When placing one sample on two plates, inoculate the non-selective medium first ! Very gently transfer your sample to the plate. You want to avoid gouging the surface of the media. Image: Arm plate, Source unknown Take your samples & place completed plates in “Save” bin. Then we will move on to the next part of lab, learning how to make a bacterial smear. From the Virtual Microbiology Classroom on ScienceProfOnline.com
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Confused? Here are links to fun resources that further explain microbiology media & culture: Media & Culture Laboratory Main Page on the Virtual Microbiology Classroom of Science Prof Online . Germs , music by Weird Al Yankovic. Video by RevLucio. Normal Flora webpage, by Douglas F. Fix. Interactive page where you can select an area of the body and learn which normal flora typically colonize that location. How to Interpret: MacConkey’s (MAC), Mannitol Salt (MSA) and Blood Agar (BAP) videos from Science Prof Online. How to Pour Bacterial Growth Media into Petri Dishes , video from Science Prof Online. Bacterial growth video and narration, YouTube, Dizzo95. . Microbial Growth & Metabolism Main Page on the Virtual Microbiology Classroom of Science Prof Online . E. coli population growth time lapse video . (You must be in PPT slideshow view to click on links.) From the Virtual Microbiology Classroom on ScienceProfOnline.com
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Are microbes intimidating you? Do yourself a favor. Use the… Virtual Microbiology Classroom ( VMC ) ! The VMC is full of resources to help you succeed, including: practice test questions review questions study guides and learning objectives You can access the VMC by going to the Science Prof Online website Images: Staph, Giant Microbes; Prokaryotic cell , Mariana Ruiz
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