Ag c wm ig go el next the ratio of the target gene in

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method (Livak and Schmittgen, 2001). AG - C wm -IG - Go , el Next, the ratio of the target gene in the treated sample can be compared relative to the untreated sample by using the expression 2016, which assumes a re efficiency. A positive 2000 value indicates an increase in gene expression, whereas a negative value reflects a decrease in expression Quantitative PCR is pwerful tool for measuring viral loads and viral replication in both human and animal models Suppose a new antiretroviral treatment (ART) has been developed to combat HIV-I viral infection lead. In order to evaluate the efficacy of this treatment before animal drug
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trials, a GPCR experiment is planned. The study will use a proxy to estimate levels of HIV-I in each sample. A reference gene is used as a baseline. The reference is usually a housekeeping one that is constitutively expressed A previous study by Watson et al. (2007) identified the one PPIA as an ideal housekeeping one or PCR and was therefore used in this study. The target gerne for HIVI was the opene, as used in Weber et al. (2003) Untreated HIV infected whole hood leukocytes are collected from a single patient and split into six experimental trials an untreated group and then the groups cach receiving a different dose of the ART in 10 mg, 20 mp. 30 m, 60 mg, and 50 mg dnes. Partial results from the PCR experiment for the HIV-1 bare gone only we shown.
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