E55-2010 exam 1

1 2 e 55 exam 1 2010 name page 8 of 8 26 12 points 2

Info iconThis preview shows pages 7–8. Sign up to view the full content.

View Full Document Right Arrow Icon
1. ____________________________________ 2. ____________________________________
Background image of page 7

Info iconThis preview has intentionally blurred sections. Sign up to view the full version.

View Full Document Right Arrow Icon
E-55, Exam 1-2010: Name Page 8 of 8 26. (12 points, 2 points each for A-C, 6 points for D) You are a graduate student in Stu Orkin’s lab at Childrens Hospital Boston, a lab that has an international reputation for studying transcription factor control of blood development. You find a novel transcription factor that is highly expressed in the blood and are very excited at the prospect of contributing to the long-standing lab legacy with your research. You rush to make a mouse knockout without any further experiments, and find that your homozygote knock-out mice die during early gestation. A. What is the most likely explanation for this result? You realize that you should you have employed some other techniques to find out more about the expression of the transcription factor during embryogenesis. B. Name two techniques that would have told you information about expression of the gene. 1. ____________________________________ 2. ____________________________________ C. Given the embryonic lethality of the knockout, what technique will you now use to knockou t the transcription factor just in the developing blood ? (Hint: You have numerous blood-specific promoters at your disposal in the Orkin lab.) ______________________________________________ D. How does this technique interfere only with blood development? Clearly describe AND ALSO draw simple diagrams to illustrate the DNA constructs that you will use to engineer the system you listed in part C.
Background image of page 8
This is the end of the preview. Sign up to access the rest of the document.

{[ snackBarMessage ]}

Page7 / 8

1 2 E 55 Exam 1 2010 Name Page 8 of 8 26 12 points 2 points...

This preview shows document pages 7 - 8. Sign up to view the full document.

View Full Document Right Arrow Icon
Ask a homework question - tutors are online