Grading is digital your patient will live and you

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Grading is Digital. Your Patient will live and you will each receive 20 Points - or - your Patient will die and you will receive 0 Points. Each Clan should work as a Team and set-up the appropriate Biochemical Tests for your Unknown Bacteria. 1. Each Clan should sign-up for an Unknown Bacterium from the Biochemical Unknown List at the Front of the Lab. 2. Your Tutor will deliver the actual Unknown Plates. Tipping is allowed. 3. Record the Code Numbers in your Lab Notebook. 4. Optional. Make a Smear of your Unknown. Heat Fix this Smear. Wrap a Piece of Tape around the Left End of this Slide and label this Tape with your Unknown Number. Give this Slide to your TA, who will save this Slide as an Archive Smear of your Unknown. 5. Perform a Gram Stain on your Unknown. It would be most unwise to neglect to use our Gram Stain Standards when you do these Gram Stains. You are strongly advised to not proceed until everyone in your Clan agrees on the Gram Stain. 6. Depending on the Gram Stain Results, perform Oxidase and/or Catalase Tests on your Unknown. You’ll want to refer to the Flow Charts at the End of Lab 8 to determine precisely which Tests you’ll be performing. • These Reagents are available at any Time on the Bench at the back of each Lab 7. Present your preliminary Results to your TA in Writing, and request the appropriate Biochemical Test Media in Writing. 8. Label all of your Tubes carefully. Put the appropriate Information onto a Piece of Tape, make a Quick Release Tab and attach the Tape to the Glass Portion of each Test Tube. 9. Inoculate these Biochemical Tests at the appropriate Temperature for the appropriate Length of Time. 10. Save your Unknown Plate at 04°C.
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Lab 7 Page 14 Background Information for Lab 7 Exercises Viable Count Look at all of your Plates but check for Plates with between 30 and 300 Colonies. Colonies can overlap and still be counted (see Figure 7-1). If a Plate contains more than 300 Colonies, make a Notation of TNTC (Too Numerous To Count.). Even 300 Colonies is more than any reasonably sane Person wants to count, so use your Sharpie to mark such Plates into Quadrants, count the Number of Colonies within a single Quadrant, and then multiply this Number by Four. You should record the Number of Colonies on Plates containing fewer than 100 Colonies, but don’t use these for your calculations, unless you have no Alternative. The number of Viable Bacteria per Gram of Hamburger will simply be equal to the number of Colonies on a Plate divided by the Dilution Factor of that Plate. If you count 150 Colonies on your “7” Spread Plate, the Number of Viable Bacteria in the Hamburger would be 150 ÷ 10 -7 = 150 x 10 7 = 1.5 x 10 9 Colony Forming Units (i.e. Bacteria) per Gram of Hamburger. (Since we’re not absolutely sure that each Colony is the Result of a Single Bacterium, we use the Term “Colony Forming Units” instead of Bacteria when reporting the Results of a Viable Count.) Compare your Results to those of other Groups.
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