# Exercise 2 calculate microscope magnification

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EXERCISE 2 – Calculate microscope magnificationCalculate the total magnification for each lens combination.Lens Lens Power Ring Color Total MagnificationUnaided Eye1Xnone 1X x 1X = 1Xeyepiece10Xnone1X x 10X = 10XScanning 4Xblack10X x4x=40xLow Power 10Xgreen10X x10x=100xHigh Power 40X yellow10X x40x=400xOil Immersion 100X red10X x100x=1000xIII. Viewing Microscope SlidesEXERCISE 3 – Focusing the microscopeDefine the following terms.ParcentralParfocal
EXERCISE 4 – Specimen orientationIn which direction does the image move?
In which direction does the image move?
What is the relationship between the movement of the image and the object?
IV.Microscopic MeasurementEXERCISE 5 – Measure the diameter of the field of viewCalculate the diameter of the field of view of the scanning power (40X) in micrometers (µm)?
Lab Report – Page 4 Calculate the size of the diameter of the field of view for each objective lens and record the data. Remember that 1mm = 1000 µm.
EXERCISE 6 – Estimate the size of a specimen1.Estimate the length of the specimen under low power (10X).
2.Measure the length of the specimen under high power (40X).
What is the relationship between the diameter of the field of view and its power of magnification?
A specimen which fills ¼ of the field of view under an oil immersion lens (1000X) is how long?
A specimen which fills ¼ of the field of view under a scanning lens (40X) is how long?
A specimen which fills ¼ of the field of view under a low power lens (100X) is how long?
A specimen which fills ¼ of the field of view under a high power lens (400X) is how long?
Lab Report – Page 5 440μmV.Depth of FocusEXERCISE 3.7 – Determine depth of focusDetermine the order of the colored threads and enter your results below:TopyellowMiddleredBottomblue