Swab moistened with saline from skin surface for aerobic bacteria should be

Swab moistened with saline from skin surface for

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Swab moistened with saline from skin surface for aerobic bacteria should be collected from the center of the wound as margins might be contaminated with normal flora. Deep wound aspirate (using needle and syringe) is optimal for recovery of anaerobes.
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Wound infection Aerobes: Staphylococcus aureus Pseudomonas Anaerobes : Clostridium perfringenes Fungi : Candida Aspergillus
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Anaerobic cultures Avoid contact with oxygen Avoid contamination with normal flora Do not refrigerate ,transport immediately Use proper transport media if delay is expected
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Important pathogens Neisseria gonorrheae, Staphylococcus aureus, Haemophilus influenzae, chlamydiae, Adeno virus Culture should be inoculated directly in the ward or clinic Conjunctiva
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Throat (Pharyngitis,Tonsilitis Main causes : Streptococcus pyogenes Other causes : S.aurues, Corynebacterium diphtheriae , Adenovirus, Rhinovirus Nurse role 6. Transport swab with transport medium 5. Avoid touching the tongue,cheeks,lips 4. Collect specimen from the both tonsils ,inflammed,ulcerative area 3. Use tongue depresser and ask patient to say AHHHHH 2. Use Proper light against patient face 1. Use sterile cotton swab
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Pulmonary Infections
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Sputum (expectorated): non sterile due to passage through the oral cavity Routine microbiology : S.pneumoniae, H.influenzae & (Pseudomonas in Cystic fibrosis patients) Special request for mycobacterium tuberculosis. Nurse role: teaching and instruction Collection of specimen: First morning sputum sample is preferred Ask patient to brush teeth, rinse his mouth with water Take a breath hold it then produce deep cough up into a wide- mouthed clean container. Place tissue around container and dispose it after sample collection. Specimen will be evaluated for squamous epithelial cells to role out contamination with saliva
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Urine culture Clean catch midstream urine sample: Nurse role : teaching /instruction the patient proper sample collection. Early morning sample, cleaning genital area with water and soap, the patient should retract the labial folds or glans penis discard the first part into the toilet and collect the midpart of stream then continue into the toilet.
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  • Fall '19
  • Bacteria, specimen collection, Wound infection, sputum,  Express

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