Swab moistened with saline from skin surface for aerobic bacteria should be collected from the center of the wound as margins might be contaminated with normal flora.Deep wound aspirate (using needle and syringe) is optimal for recovery of anaerobes.
Anaerobic culturesAvoid contact with oxygenAvoid contamination with normal floraDo not refrigerate ,transport immediatelyUse proper transport media if delay is expected
Important pathogensNeisseria gonorrheae,Staphylococcus aureus,Haemophilus influenzae, chlamydiae, Adeno virusCulture should be inoculated directly in the ward or clinic Conjunctiva
Throat (Pharyngitis,TonsilitisMain causes : Streptococcus pyogenesOther causes : S.aurues, Corynebacterium diphtheriae , Adenovirus, RhinovirusNurse role6. Transport swab with transport medium5. Avoid touching the tongue,cheeks,lips4. Collect specimen from the both tonsils ,inflammed,ulcerative area3. Use tongue depresser and ask patient to say AHHHHH2. Use Proper light against patient face1. Use sterile cotton swab
Sputum (expectorated): non sterile due to passage through the oral cavityRoutine microbiology : S.pneumoniae, H.influenzae& (Pseudomonas in Cystic fibrosis patients)Special request for mycobacterium tuberculosis.Nurse role: teaching and instructionCollection of specimen: First morning sputum sample is preferredAsk patient to brush teeth, rinse his mouth with water Take a breath hold it then produce deep cough up into a wide-mouthed clean container. Place tissue around container and dispose it after sample collection.Specimen will be evaluated for squamous epithelial cells to role out contamination with saliva
Urine cultureClean catch midstream urine sample: Nurse role: teaching/instruction the patient proper sample collection.Early morning sample, cleaning genital area with water and soap, the patient should retract the labial folds or glans penis discard the first part into the toilet and collect the midpart of stream then continue into the toilet.
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