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5.Using the dilution equation (below) to solve for your needed volume of stock solution (V1), make an additional 200 mL of 15% sucrose, and pour this into beaker 1.
6. Pour 5 ml (measure this with the graduated cylinder) of the three solutions you prepared in step 4 into three of your prepared dialysis bags as follows:Pour the 30% solution into the bag with the blue rubber band. Pour the 15% solution into the bag with the red rubber band. Pour the 3% solution into the bag with the yellow rubber band. Finally, pour 5 mls of one of the sugar-containing liquids you chose (juice, soda, etc.) intothe dialysis bag with the green rubber band. Pour 5 mL of the second sugar-containing liquid you chose into the dialysis bag with the white rubber band.Secure the tops of each bag in the same way that you secured the bottoms of each bag, using the same colored rubber bands for each bag (if you don’t have enough tubing, you don’t have to knot the tops of the bags, but make sure the tops are as secure as possible using the folding/rubber band method).IMPORTANT:Your “unknown” liquids should be at room temperature when you pour them into the dialysis bag.IMPORTANT:Make sure when you secure the tops of the dialysis tubing to leave empty space above the liquids in the tubes to leave space for any water that may move into thebag during your experiment!Describe your “unknown” sugar solutions here.For example, you could say, “I dissolved1 tablespoon (~15 mL) of honey in 100 mL tap water, and poured 5 mL of this into one dialysis bag, and I poured 5 mL of room temperature orange juice into the other dialysis bag.” I dissolved ½ tablespoon honey into 4 ounces of hot water and let it come to room temperature. I poured 5 mL of the honey/water solution into the dialysis bag with green rubber band, and 5 mL of soda into the other bag (no white rubber bands in kit, so I just tied the bag ends).In addition, take a photograph of your experimental set-up (the dialysis bags in the beaker), and insert it here.